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@article{1412746,
author = {Hrbáč, Jan and Novák, David and Knopf, Petr and Švarc, Marek and Vacek, Jan},
article_location = {Lausanne},
article_number = {April 1},
doi = {http://dx.doi.org/10.1016/j.jelechem.2018.02.037},
keywords = {Carbon fiber Microelectrode On-line sensing Amperometry Clinical sample Trolox},
language = {eng},
issn = {1572-6657},
journal = {Journal of Electroanalytical Chemistry},
title = {Carbon fiber on-line detector for monitoring human blood serum reductive capacity. A complex technical solution},
url = {http://dx.doi.org/10.1016/j.jelechem.2018.02.037},
volume = {814},
year = {2018}
}
TY - JOUR
ID - 1412746
AU - Hrbáč, Jan - Novák, David - Knopf, Petr - Švarc, Marek - Vacek, Jan
PY - 2018
TI - Carbon fiber on-line detector for monitoring human blood serum reductive capacity. A complex technical solution
JF - Journal of Electroanalytical Chemistry
VL - 814
IS - April 1
SP - 184-191
EP - 184-191
PB - Elsevier
SN - 15726657
KW - Carbon fiber Microelectrode On-line sensing Amperometry Clinical sample Trolox
UR - http://dx.doi.org/10.1016/j.jelechem.2018.02.037
N2 - The construction of a cylindrical carbon fiber on-line detector is presented, featuring a novel way of sealing the carbon fiber microelectrode, the design of an interchangeable carbon fiber microelectrode-based flow cell and its interface with data acquisition electronics. The two-electrode setup was used, where the regeneration of the carbon fiber working electrode surface between analyses was performed using an electrochemical procedure, typically -1/+1V potential cycling. The detector was incorporated into the flow injection analysis manifold and the applicability of the developed sensing platform tested for monitoring a hexacyanoferrate (II/III) redox probe and the oxidizable compounds trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), uric acid, and l-ascorbic acid, with dehydroascorbic acid as a negative control. Finally, the optimized procedure was utilized for the analysis of human blood serum reductive (antioxidant) capacity based on amperometric sensing and flow injection analysis at a flow rate of 10ul/min. Amperometric responses for blood serum samples were evaluated for standard deviation, whose average value was estimated to be ±0.3nA (n=20). The electrolytic efficiency of the detector was on the order of % units, e.g. 2% for trolox. The flow cell geometry and application of carbon fiber electrodes resulted in the absence of dead volumes and passivation phenomena, in contrast to conventional high-surface-area electrochemical cells. We suppose that the complex technical solution presented here could find a broad range of applications in the on-line monitoring of other reducing low-molecular bioactives and of the redox properties of various body fluids or clinical samples.
ER -
HRBÁČ, Jan, David NOVÁK, Petr KNOPF, Marek ŠVARC a Jan VACEK. Carbon fiber on-line detector for monitoring human blood serum reductive capacity. A complex technical solution. Online. \textit{Journal of Electroanalytical Chemistry}. Lausanne: Elsevier, 2018, roč.~814, April 1, s.~184-191. ISSN~1572-6657. Dostupné z: https://dx.doi.org/10.1016/j.jelechem.2018.02.037. [citováno 2024-04-24]
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