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@article{1415268, author = {Ibrahim, Emad and Dobeš, Pavel and Kunc, Martin and Hyršl, Pavel and Kodrík, Dalibor}, article_location = {Kidlington, England}, article_number = {4}, doi = {http://dx.doi.org/10.1016/j.jinsphys.2018.04.002}, keywords = {Adipokinetic hormone; adenosine; Drosophila; nematode; oxidative stress; locomotion}, language = {eng}, issn = {0022-1910}, journal = {Journal of Insect Physiology}, title = {Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster}, url = {http://dx.doi.org/10.1016/j.jinsphys.2018.04.002}, volume = {107}, year = {2018} }
TY - JOUR ID - 1415268 AU - Ibrahim, Emad - Dobeš, Pavel - Kunc, Martin - Hyršl, Pavel - Kodrík, Dalibor PY - 2018 TI - Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster JF - Journal of Insect Physiology VL - 107 IS - 4 SP - 167-174 EP - 167-174 PB - Elsevier SN - 00221910 KW - Adipokinetic hormone KW - adenosine KW - Drosophila KW - nematode KW - oxidative stress KW - locomotion UR - http://dx.doi.org/10.1016/j.jinsphys.2018.04.002 N2 - This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh1, AdoR1 (adenosine receptor), and Akh1 AdoR1. Mortality decreased in all mutants post-EPN infection compared with the control (w1118). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w1118 and Akh1 larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR1 and Akh1 AdoR1 mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh1. In uninfected larvae mutations decreased antioxidative capacity in Akh1 and increased in AdoR1, however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w1118 larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion. ER -
IBRAHIM, Emad, Pavel DOBEŠ, Martin KUNC, Pavel HYRŠL and Dalibor KODRÍK. Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster. \textit{Journal of Insect Physiology}. Kidlington, England: Elsevier, 2018, vol.~107, No~4, p.~167-174. ISSN~0022-1910. Available from: https://dx.doi.org/10.1016/j.jinsphys.2018.04.002.
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