JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Lukas RICHTERA, Ludmila KREJCOVA, Lukas KALINA, Rahul DATTA, Markéta SVOBODOVÁ, David HYNEK, Michal MASAŘÍK, Zbynek HEGER and Vojtech ADAM. Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection. Sensors and Actuators B: Chemical. Lausanne: Elsevier, 2018, vol. 258, APR 1 2018, p. 295-303. ISSN 0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2017.11.074. |
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@article{1417178, author = {Jimenez, Ana Maria Jimenez and Moulick, Amitava and Richtera, Lukas and Krejcova, Ludmila and Kalina, Lukas and Datta, Rahul and Svobodová, Markéta and Hynek, David and Masařík, Michal and Heger, Zbynek and Adam, Vojtech}, article_location = {Lausanne}, article_number = {APR 1 2018}, doi = {http://dx.doi.org/10.1016/j.snb.2017.11.074}, keywords = {Electrochemistry; Human immunodeficiency virus; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots}, language = {eng}, issn = {0925-4005}, journal = {Sensors and Actuators B: Chemical}, title = {Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection}, volume = {258}, year = {2018} }
TY - JOUR ID - 1417178 AU - Jimenez, Ana Maria Jimenez - Moulick, Amitava - Richtera, Lukas - Krejcova, Ludmila - Kalina, Lukas - Datta, Rahul - Svobodová, Markéta - Hynek, David - Masařík, Michal - Heger, Zbynek - Adam, Vojtech PY - 2018 TI - Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection JF - Sensors and Actuators B: Chemical VL - 258 IS - APR 1 2018 SP - 295-303 EP - 295-303 PB - Elsevier SN - 09254005 KW - Electrochemistry KW - Human immunodeficiency virus KW - Human papillomavirus KW - Nanotechnology KW - Magnetic isolation KW - Quantum dots N2 - Human papillomavirus (HPV) and human immunodeficiency virus (HIV) are closely related infections; both diseases are mutually reinforcing due to the co-infection effects, which increase their clinical and epidemiological implications, raising urgent public health concerns. In the present study, a nano-system was developed for simultaneous detection of HPV and HIV targeting E6-HPV16 and Gag-HIV genes respectively. The target sequences from the samples of the patients suffering from head and neck cancer were isolated by magnetic glass particles (MGPs) and subsequently were hybridized with a mixture of the complementary probes attached to cadmium telluride quantum dots (CdTe QDs). Finally, the specific and successful binding was determined by an easy fluorescence analyses and was corroborated by PCR and electrochemistry. Red and green CdTe QDs were used for the dual-color detection of HPV and HIV respectively. Our nano-system can be applied as a proficient tool for the identification of HPV, HIV or both diseases simultaneously, getting a successful and high sensitivity system of detection. (C) 2017 Elsevier B.V. All rights reserved. ER -
JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Lukas RICHTERA, Ludmila KREJCOVA, Lukas KALINA, Rahul DATTA, Markéta SVOBODOVÁ, David HYNEK, Michal MASAŘÍK, Zbynek HEGER and Vojtech ADAM. Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection. \textit{Sensors and Actuators B: Chemical}. Lausanne: Elsevier, 2018, vol.~258, APR 1 2018, p.~295-303. ISSN~0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2017.11.074.
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