Dual-color quantum dots-based simultaneous detection of HPV-HIV
co-infection

J 2018

Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Lukas RICHTERA, Ludmila KREJCOVA, Lukas KALINA et. al.

Basic information

Original name

Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection

Authors

JIMENEZ, Ana Maria Jimenez (203 Czech Republic), Amitava MOULICK (203 Czech Republic), Lukas RICHTERA (203 Czech Republic), Ludmila KREJCOVA (203 Czech Republic), Lukas KALINA (203 Czech Republic), Rahul DATTA (826 United Kingdom of Great Britain and Northern Ireland), Markéta SVOBODOVÁ (203 Czech Republic, belonging to the institution), David HYNEK (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Zbynek HEGER (203 Czech Republic) and Vojtech ADAM (203 Czech Republic, guarantor)

Edition

Sensors and Actuators B: Chemical, Lausanne, Elsevier, 2018, 0925-4005

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 6.393

RIV identification code

RIV/00216224:14110/18:00102718

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.snb.2017.11.074

UT WoS

000424868700033

Keywords in English

Electrochemistry; Human immunodeficiency virus; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots

Abstract

V originále

Human papillomavirus (HPV) and human immunodeficiency virus (HIV) are closely related infections; both diseases are mutually reinforcing due to the co-infection effects, which increase their clinical and epidemiological implications, raising urgent public health concerns. In the present study, a nano-system was developed for simultaneous detection of HPV and HIV targeting E6-HPV16 and Gag-HIV genes respectively. The target sequences from the samples of the patients suffering from head and neck cancer were isolated by magnetic glass particles (MGPs) and subsequently were hybridized with a mixture of the complementary probes attached to cadmium telluride quantum dots (CdTe QDs). Finally, the specific and successful binding was determined by an easy fluorescence analyses and was corroborated by PCR and electrochemistry. Red and green CdTe QDs were used for the dual-color detection of HPV and HIV respectively. Our nano-system can be applied as a proficient tool for the identification of HPV, HIV or both diseases simultaneously, getting a successful and high sensitivity system of detection. (C) 2017 Elsevier B.V. All rights reserved.

Citovat

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Lukas RICHTERA, Ludmila KREJCOVA, Lukas KALINA, Rahul DATTA, Markéta SVOBODOVÁ, David HYNEK, Michal MASAŘÍK, Zbynek HEGER and Vojtech ADAM. Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection. Sensors and Actuators B: Chemical. Lausanne: Elsevier, 2018, vol. 258, APR 1 2018, p. 295-303. ISSN 0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2017.11.074.

@article{1417178,
   author = {Jimenez, Ana Maria Jimenez and Moulick, Amitava and Richtera, Lukas and Krejcova, Ludmila and Kalina, Lukas and Datta, Rahul and Svobodová, Markéta and Hynek, David and Masařík, Michal and Heger, Zbynek and Adam, Vojtech},
   article_location = {Lausanne},
   article_number = {APR 1 2018},
   doi = {http://dx.doi.org/10.1016/j.snb.2017.11.074},
   keywords = {Electrochemistry; Human immunodeficiency virus; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots},
   language = {eng},
   issn = {0925-4005},
   journal = {Sensors and Actuators B: Chemical},
   title = {Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection},
   volume = {258},
   year = {2018}
}

TY  - JOUR
ID  - 1417178
AU  - Jimenez, Ana Maria Jimenez - Moulick, Amitava - Richtera, Lukas - Krejcova, Ludmila - Kalina, Lukas - Datta, Rahul - Svobodová, Markéta - Hynek, David - Masařík, Michal - Heger, Zbynek - Adam, Vojtech
PY  - 2018
TI  - Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection
JF  - Sensors and Actuators B: Chemical
VL  - 258
IS  - APR 1 2018
SP  - 295-303
EP  - 295-303
PB  - Elsevier
SN  - 09254005
KW  - Electrochemistry
KW  - Human immunodeficiency virus
KW  - Human papillomavirus
KW  - Nanotechnology
KW  - Magnetic isolation
KW  - Quantum dots
N2  - Human papillomavirus (HPV) and human immunodeficiency virus (HIV) are closely related infections; both diseases are mutually reinforcing due to the co-infection effects, which increase their clinical and epidemiological implications, raising urgent public health concerns. In the present study, a nano-system was developed for simultaneous detection of HPV and HIV targeting E6-HPV16 and Gag-HIV genes respectively. The target sequences from the samples of the patients suffering from head and neck cancer were isolated by magnetic glass particles (MGPs) and subsequently were hybridized with a mixture of the complementary probes attached to cadmium telluride quantum dots (CdTe QDs). Finally, the specific and successful binding was determined by an easy fluorescence analyses and was corroborated by PCR and electrochemistry. Red and green CdTe QDs were used for the dual-color detection of HPV and HIV respectively. Our nano-system can be applied as a proficient tool for the identification of HPV, HIV or both diseases simultaneously, getting a successful and high sensitivity system of detection. (C) 2017 Elsevier B.V. All rights reserved.
ER  -

JIMENEZ, Ana Maria Jimenez, Amitava MOULICK, Lukas RICHTERA, Ludmila KREJCOVA, Lukas KALINA, Rahul DATTA, Markéta SVOBODOVÁ, David HYNEK, Michal MASAŘÍK, Zbynek HEGER and Vojtech ADAM. Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection. \textit{Sensors and Actuators B: Chemical}. Lausanne: Elsevier, 2018, vol.~258, APR 1 2018, p.~295-303. ISSN~0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2017.11.074.

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