Detailed Information on Publication Record
2018
Computer-assisted engineering of hyperstable fibroblast growth factor 2
DVOŘÁK, Pavel, David BEDNÁŘ, Pavel VAŇÁČEK, Lukáš BÁLEK, Lívia EISELLEOVÁ et. al.Basic information
Original name
Computer-assisted engineering of hyperstable fibroblast growth factor 2
Authors
DVOŘÁK, Pavel (203 Czech Republic, belonging to the institution), David BEDNÁŘ (203 Czech Republic, belonging to the institution), Pavel VAŇÁČEK (203 Czech Republic, belonging to the institution), Lukáš BÁLEK (203 Czech Republic, belonging to the institution), Lívia EISELLEOVÁ (703 Slovakia, belonging to the institution), Veronika ŠTĚPÁNKOVÁ (203 Czech Republic, belonging to the institution), Eva ŠEBESTOVÁ (203 Czech Republic, belonging to the institution), Michaela BOSÁKOVÁ (203 Czech Republic, belonging to the institution), Žaneta KONEČNÁ (203 Czech Republic, belonging to the institution), Stanislav MAZURENKO (643 Russian Federation, belonging to the institution), Antonín KUNKA (203 Czech Republic, belonging to the institution), Tereza VÁŇOVÁ (203 Czech Republic, belonging to the institution), Karolína ZOUFALOVÁ (203 Czech Republic, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Pavel KREJČÍ (203 Czech Republic, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution), Petr DVOŘÁK (203 Czech Republic, belonging to the institution) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Biotechnology and Bioengineering, New York, Wiley, 2018, 0006-3592
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
20800 2.8 Environmental biotechnology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.260
RIV identification code
RIV/00216224:14310/18:00100936
Organization unit
Faculty of Science
UT WoS
000426493300005
Keywords in English
computational design; embryonic stem cells; fibroblast growth factor; focused directed evolution; protein engineering; protein stability
Tags
International impact, Reviewed
Změněno: 30/4/2019 08:58, Mgr. Tereza Miškechová
Abstract
V originále
Fibroblast growth factors (FGFs) serve numerous regulatory functions in complex organisms, and their corresponding therapeutic potential is of growing interest to academics and industrial researchers alike. However, applications of these proteins are limited due to their low stability. Here we tackle this problem using a generalizable computer-assisted protein engineering strategy to create a unique modified FGF2 with nine mutations displaying unprecedented stability and uncompromised biological function. The data from the characterization of stabilized FGF2 showed a remarkable prediction potential of in silico methods and provided insight into the unfolding mechanism of the protein. The molecule holds a considerable promise for stem cell research and medical or pharmaceutical applications.
Links
| GA15-23033S, research and development project |
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| GA16-06096S, research and development project |
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| LM2015047, research and development project |
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| LM2015051, research and development project |
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| LM2015055, research and development project |
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| LM2015085, research and development project |
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| LO1214, research and development project |
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