J 2018

Computer-assisted engineering of hyperstable fibroblast growth factor 2

DVOŘÁK, Pavel, David BEDNÁŘ, Pavel VAŇÁČEK, Lukáš BÁLEK, Lívia EISELLEOVÁ et. al.

Basic information

Original name

Computer-assisted engineering of hyperstable fibroblast growth factor 2

Authors

DVOŘÁK, Pavel (203 Czech Republic, belonging to the institution), David BEDNÁŘ (203 Czech Republic, belonging to the institution), Pavel VAŇÁČEK (203 Czech Republic, belonging to the institution), Lukáš BÁLEK (203 Czech Republic, belonging to the institution), Lívia EISELLEOVÁ (703 Slovakia, belonging to the institution), Veronika ŠTĚPÁNKOVÁ (203 Czech Republic, belonging to the institution), Eva ŠEBESTOVÁ (203 Czech Republic, belonging to the institution), Michaela BOSÁKOVÁ (203 Czech Republic, belonging to the institution), Žaneta KONEČNÁ (203 Czech Republic, belonging to the institution), Stanislav MAZURENKO (643 Russian Federation, belonging to the institution), Antonín KUNKA (203 Czech Republic, belonging to the institution), Tereza VÁŇOVÁ (203 Czech Republic, belonging to the institution), Karolína ZOUFALOVÁ (203 Czech Republic, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Pavel KREJČÍ (203 Czech Republic, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution), Petr DVOŘÁK (203 Czech Republic, belonging to the institution) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biotechnology and Bioengineering, New York, Wiley, 2018, 0006-3592

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20800 2.8 Environmental biotechnology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

One Library Wiley

Impact factor

Impact factor: 4.260

RIV identification code

RIV/00216224:14310/18:00100936

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/bit.26531

UT WoS

000426493300005

Keywords in English

computational design; embryonic stem cells; fibroblast growth factor; focused directed evolution; protein engineering; protein stability

Tags

14110513, NZ, podil, rivok

Tags

International impact, Reviewed
Změněno: 30/4/2019 08:58, Mgr. Tereza Miškechová

Abstract

V originále

Fibroblast growth factors (FGFs) serve numerous regulatory functions in complex organisms, and their corresponding therapeutic potential is of growing interest to academics and industrial researchers alike. However, applications of these proteins are limited due to their low stability. Here we tackle this problem using a generalizable computer-assisted protein engineering strategy to create a unique modified FGF2 with nine mutations displaying unprecedented stability and uncompromised biological function. The data from the characterization of stabilized FGF2 showed a remarkable prediction potential of in silico methods and provided insight into the unfolding mechanism of the protein. The molecule holds a considerable promise for stem cell research and medical or pharmaceutical applications.

Links

GA15-23033S, research and development project
Name: Úloha sekrece FGF2 ve fyziologii lidských pluripotentních kmenových buněk
Investor: Czech Science Foundation
GA16-06096S, research and development project
Name: Objasnění významu dynamických tunelů pro enzymatickou katalýzu: simulace a fluorescenční experimenty
Investor: Czech Science Foundation
LM2015047, research and development project
Name: Česká národní infrastruktura pro biologická data (Acronym: ELIXIR-CZ)
Investor: Ministry of Education, Youth and Sports of the CR, Czech National Infrastructure for Biological Data
LM2015051, research and development project
Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR
LM2015055, research and development project
Name: Centrum pro systémovou biologii (Acronym: C4SYS)
Investor: Ministry of Education, Youth and Sports of the CR
LM2015085, research and development project
Name: CERIT Scientific Cloud (Acronym: CERIT-SC)
Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud
LO1214, research and development project
Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)
Investor: Ministry of Education, Youth and Sports of the CR
Displayed: 4/11/2024 06:38