| DVOŘÁK, Pavel, David BEDNÁŘ, Pavel VAŇÁČEK, Lukáš BÁLEK, Lívia EISELLEOVÁ, Veronika ŠTĚPÁNKOVÁ, Eva ŠEBESTOVÁ, Michaela BOSÁKOVÁ, Žaneta KONEČNÁ, Stanislav MAZURENKO, Antonín KUNKA, Tereza VÁŇOVÁ, Karolína ZOUFALOVÁ, Radka CHALOUPKOVÁ, Jan BREZOVSKÝ, Pavel KREJČÍ, Zbyněk PROKOP, Petr DVOŘÁK and Jiří DAMBORSKÝ. Computer-assisted engineering of hyperstable fibroblast growth factor 2. Biotechnology and Bioengineering. New York: Wiley, 2018, vol. 115, No 4, p. 850-862. ISSN 0006-3592. Available from: https://dx.doi.org/10.1002/bit.26531. |
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@article{1417222,
author = {Dvořák, Pavel and Bednář, David and Vaňáček, Pavel and Bálek, Lukáš and Eiselleová, Lívia and Štěpánková, Veronika and Šebestová, Eva and Bosáková, Michaela and Konečná, Žaneta and Mazurenko, Stanislav and Kunka, Antonín and Váňová, Tereza and Zoufalová, Karolína and Chaloupková, Radka and Brezovský, Jan and Krejčí, Pavel and Prokop, Zbyněk and Dvořák, Petr and Damborský, Jiří},
article_location = {New York},
article_number = {4},
doi = {http://dx.doi.org/10.1002/bit.26531},
keywords = {computational design; embryonic stem cells; fibroblast growth factor; focused directed evolution; protein engineering; protein stability},
language = {eng},
issn = {0006-3592},
journal = {Biotechnology and Bioengineering},
title = {Computer-assisted engineering of hyperstable fibroblast growth factor 2},
url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/bit.26531},
volume = {115},
year = {2018}
}
TY - JOUR
ID - 1417222
AU - Dvořák, Pavel - Bednář, David - Vaňáček, Pavel - Bálek, Lukáš - Eiselleová, Lívia - Štěpánková, Veronika - Šebestová, Eva - Bosáková, Michaela - Konečná, Žaneta - Mazurenko, Stanislav - Kunka, Antonín - Váňová, Tereza - Zoufalová, Karolína - Chaloupková, Radka - Brezovský, Jan - Krejčí, Pavel - Prokop, Zbyněk - Dvořák, Petr - Damborský, Jiří
PY - 2018
TI - Computer-assisted engineering of hyperstable fibroblast growth factor 2
JF - Biotechnology and Bioengineering
VL - 115
IS - 4
SP - 850-862
EP - 850-862
PB - Wiley
SN - 00063592
KW - computational design
KW - embryonic stem cells
KW - fibroblast growth factor
KW - focused directed evolution
KW - protein engineering
KW - protein stability
UR - https://onlinelibrary.wiley.com/doi/epdf/10.1002/bit.26531
L2 - https://onlinelibrary.wiley.com/doi/epdf/10.1002/bit.26531
N2 - Fibroblast growth factors (FGFs) serve numerous regulatory functions in complex organisms, and their corresponding therapeutic potential is of growing interest to academics and industrial researchers alike. However, applications of these proteins are limited due to their low stability. Here we tackle this problem using a generalizable computer-assisted protein engineering strategy to create a unique modified FGF2 with nine mutations displaying unprecedented stability and uncompromised biological function. The data from the characterization of stabilized FGF2 showed a remarkable prediction potential of in silico methods and provided insight into the unfolding mechanism of the protein. The molecule holds a considerable promise for stem cell research and medical or pharmaceutical applications.
ER -
DVOŘÁK, Pavel, David BEDNÁŘ, Pavel VAŇÁČEK, Lukáš BÁLEK, Lívia EISELLEOVÁ, Veronika ŠTĚPÁNKOVÁ, Eva ŠEBESTOVÁ, Michaela BOSÁKOVÁ, Žaneta KONEČNÁ, Stanislav MAZURENKO, Antonín KUNKA, Tereza VÁŇOVÁ, Karolína ZOUFALOVÁ, Radka CHALOUPKOVÁ, Jan BREZOVSKÝ, Pavel KREJČÍ, Zbyněk PROKOP, Petr DVOŘÁK and Jiří DAMBORSKÝ. Computer-assisted engineering of hyperstable fibroblast growth factor 2. \textit{Biotechnology and Bioengineering}. New York: Wiley, 2018, vol.~115, No~4, p.~850-862. ISSN~0006-3592. Available from: https://dx.doi.org/10.1002/bit.26531.
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