Prussian blue nanoparticles as nanozyme label in immunoassays

ČUNDERLOVÁ, Veronika, Antonín HLAVÁČEK, Zdeněk FARKA, Veronika HORÁČKOVÁ, Zuzana MIKUŠOVÁ and Petr SKLÁDAL. Prussian blue nanoparticles as nanozyme label in immunoassays. In CEITEC PhD and Postdoc Retreat. 2018. ISBN 978-80-210-8941-9.

Basic information

• Original name: Prussian blue nanoparticles as nanozyme label in immunoassays
• Authors: ČUNDERLOVÁ, Veronika (203 Czech Republic, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution), Veronika HORÁČKOVÁ (203 Czech Republic, belonging to the institution), Zuzana MIKUŠOVÁ (703 Slovakia, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: CEITEC PhD and Postdoc Retreat, 2018.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/18:00102730
• Organization unit: Central European Institute of Technology
• ISBN: 978-80-210-8941-9
• Keywords in English: Prussian blue nanoparticle; Enzyme biomimic; Bioconjugation; Sandwich immunoassay; Salmonella; Human serum albumin
• Tags: rivok

Abstract

Enzymes represent the most often used labels for amplification of signal in immunoassays due to their high catalytic efficiency. However, natural enzymes suffer from several disadvantages, e.g. they are easily denatured and their production and purification is generally complicated and time-consuming. Contrary, inorganic catalytic nanoparti-cles (nanozymes) offer easy and fast synthesis from commonly used chemicals. One of them are Prussian blue nanoparticles (PBNPs), which are nanozymes composed of coordination polymer of Fe4[Fe(CN)6]3. PBNPs contain the Fe3+/Fe2+ redox couple on the surface, which is also responsible for catalytic activity of peroxidases. PBNPs show high efficiency in catalyzing the oxidation of colorless 3,3,5,5-tetramethylbenzi-dine in the presence of H2O2 to form intensively blue product. We have prepared PBNPs with turnover number of 20 000 s-1 towards TMB oxidation, which is the highest value reported for nanoparticles of a size comparable to common proteins. We have also introduced the method for conjugation of PBNPs with antibody and utilization of the conjugate as a catalytic label in sandwich nanozyme-linked immunosorbent assay (NLISA). The practical applicability of NLISA was demonstrated by detection of different antigens, human serum albumin in urine and Salmonella Typhimurium in milk with limit of detection of 1.2 ng mL1 and 6·10^3 CFU mL1 respectively.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR