Automatic detection of laser-induced structures in live cell
fluorescent microscopy images using snakes with geometric
constraints

D 2017

Automatic detection of laser-induced structures in live cell fluorescent microscopy images using snakes with geometric constraints

KONDRATĚV, Alexandr and Dmitry SOROKIN

Basic information

Original name

Automatic detection of laser-induced structures in live cell fluorescent microscopy images using snakes with geometric constraints

Authors

KONDRATĚV, Alexandr (643 Russian Federation) and Dmitry SOROKIN (643 Russian Federation, belonging to the institution)

Edition

Cancun, Mexico, 23rd International Conference on Pattern Recognition, ICPR 2016, p. 331-336, 6 pp. 2017

Publisher

Institute of Electrical and Electronics Engineers Inc.

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10201 Computer sciences, information science, bioinformatics

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

RIV identification code

RIV/00216224:14330/17:00100647

Organization unit

Faculty of Informatics

ISBN

978-1-5090-4847-2

ISSN

DOI

http://dx.doi.org/10.1109/ICPR.2016.7899655

UT WoS

000406771300058

Keywords in English

Argon lasers; Cells; Cytology; Fluorescence; Geometry; Image registration; Image segmentation; Matrix algebra

Abstract

V originále

The existence of reliable evaluation datasets for cell image registration algorithms is crucial for quantitative comparison of registration approaches. A new technique for creating real live cell image sequences for this purpose was introduced recently. These datasets contain stable structures bleached by argon laser in the cell nucleus. In this work, we propose an approach for automatic detection of laser-induced linear structures in live cell fluorescent microscopy images. Compared to a previous linear laser-induced structure detection approach, our method employs an active contours model with a Hessian-based image energy term for linear structures enhancement and geometric energy term controlling the geometric relations between the structures. It uses position adaptive tension parameter values to adjust the snakes behavior in problematic regions (end points and intersection points) and a temporal consistent scheme where the results from the previous frame are used as an initial approximation for the current frame. Our approach was successfully applied to real live cell microscopy image sequences and an experimental comparison with an existing laser-induced structures detection method based on minimal paths has been performed.

Citovat

KONDRATĚV, Alexandr and Dmitry SOROKIN. Automatic detection of laser-induced structures in live cell fluorescent microscopy images using snakes with geometric constraints. In 23rd International Conference on Pattern Recognition, ICPR 2016. Cancun, Mexico: Institute of Electrical and Electronics Engineers Inc., 2017, p. 331-336. ISBN 978-1-5090-4847-2. Available from: https://dx.doi.org/10.1109/ICPR.2016.7899655.

@inproceedings{1417431,
   author = {Kondratěv, Alexandr and Sorokin, Dmitry},
   address = {Cancun, Mexico},
   booktitle = {23rd International Conference on Pattern Recognition, ICPR 2016},
   doi = {http://dx.doi.org/10.1109/ICPR.2016.7899655},
   keywords = {Argon lasers; Cells; Cytology; Fluorescence; Geometry; Image registration; Image segmentation; Matrix algebra},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Cancun, Mexico},
   isbn = {978-1-5090-4847-2},
   pages = {331-336},
   publisher = {Institute of Electrical and Electronics Engineers Inc.},
   title = {Automatic detection of laser-induced structures in live cell fluorescent microscopy images using snakes with geometric constraints},
   year = {2017}
}

TY  - JOUR
ID  - 1417431
AU  - Kondratěv, Alexandr - Sorokin, Dmitry
PY  - 2017
TI  - Automatic detection of laser-induced structures in live cell fluorescent microscopy images using snakes with geometric constraints
PB  - Institute of Electrical and Electronics Engineers Inc.
CY  - Cancun, Mexico
SN  - 9781509048472
KW  - Argon lasers
KW  - Cells
KW  - Cytology
KW  - Fluorescence
KW  - Geometry
KW  - Image registration
KW  - Image segmentation
KW  - Matrix algebra
N2  - The existence of reliable evaluation datasets for cell image registration algorithms is crucial for quantitative comparison of registration approaches. A new technique for creating real live cell image sequences for this purpose was introduced recently. These datasets contain stable structures bleached by argon laser in the cell nucleus. In this work, we propose an approach for automatic detection of laser-induced linear structures in live cell fluorescent microscopy images. Compared to a previous linear laser-induced structure detection approach, our method employs an active contours model with a Hessian-based image energy term for linear structures enhancement and geometric energy term controlling the geometric relations between the structures. It uses position adaptive tension parameter values to adjust the snakes behavior in problematic regions (end points and intersection points) and a temporal consistent scheme where the results from the previous frame are used as an initial approximation for the current frame. Our approach was successfully applied to real live cell microscopy image sequences and an experimental comparison with an existing laser-induced structures detection method based on minimal paths has been performed.
ER  -

KONDRATĚV, Alexandr and Dmitry SOROKIN. Automatic detection of laser-induced structures in live cell fluorescent microscopy images using snakes with geometric constraints. In \textit{23rd International Conference on Pattern Recognition, ICPR 2016}. Cancun, Mexico: Institute of Electrical and Electronics Engineers Inc., 2017, p.~331-336. ISBN~978-1-5090-4847-2. Available from: https://dx.doi.org/10.1109/ICPR.2016.7899655.

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