Amperometric immunosensor for diagnosis of albuminuria

a 2018

Amperometric immunosensor for diagnosis of albuminuria

MIKUŠOVÁ, Zuzana, Matěj PASTUCHA, Zdeněk FARKA, Veronika ČUNDERLOVÁ, Petr SKLÁDAL et. al.

Basic information

Original name

Amperometric immunosensor for diagnosis of albuminuria

Authors

MIKUŠOVÁ, Zuzana (703 Slovakia, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution), Veronika ČUNDERLOVÁ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

CEITEC PhD and Postdoc Retreat, 2018

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14740/18:00102777

Organization unit

Central European Institute of Technology

ISBN

978-80-210-8941-9

Keywords in English

Biosensor; Amperometry; Sandwich assay; Human serum albumin; Albuminuria

Abstract

V originále

Human serum albumin (HSA) is a plasma protein involved in transportation of ligands to specific targets. Its increased presence in urine (microalbuminuria) is considered as an indication of diabetic nephropathy. Currently, HSA is being typically detected by immunoassays such as ELISA. These methods provide high sensitivity; however, they are time consuming, complicated and expensive. One of the possibilities to over-come these disadvantages is to use electrochemical biosensors that offer operational simplicity, short analysis times and miniaturization possibilities for point-of-care diagnosis. The electrochemical biosensors are typically based on screen-printed electrodes (SPEs) due to their low cost, portability and mass production capabilities. We have developed an amperometric immunosensor for rapid diagnostics of albumi-nuria. Anti-HSA antibody was immobilized to the surface of SPEs that allowed specific capture of HSA. The detection was based on binding of secondary antibody conjugated with horseradish peroxidase (HRP), that provides oxidation of 3,3´,5,5´-tetramethylbenzidine (TMB) in presence of H2O2. Alternatively, Prussian blue catalytic nanoparticles were employed as convenient enzyme replacement. SPEs based on gold and carbon (including carbon nanotubes) working electrode were compared. This method has proved to be suitable for determination of clinically relevant concentrations of HSA in urine, optimization focused on better detection limit is in progress.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MIKUŠOVÁ, Zuzana, Matěj PASTUCHA, Zdeněk FARKA, Veronika ČUNDERLOVÁ and Petr SKLÁDAL. Amperometric immunosensor for diagnosis of albuminuria. In CEITEC PhD and Postdoc Retreat. 2018. ISBN 978-80-210-8941-9.

@proceedings{1418321,
   author = {Mikušová, Zuzana and Pastucha, Matěj and Farka, Zdeněk and Čunderlová, Veronika and Skládal, Petr},
   booktitle = {CEITEC PhD and Postdoc Retreat},
   keywords = {Biosensor; Amperometry; Sandwich assay; Human serum albumin; Albuminuria},
   language = {eng},
   isbn = {978-80-210-8941-9},
   title = {Amperometric immunosensor for diagnosis of albuminuria},
   url = {http://phdretreat.ceitec.cz/download/174},
   year = {2018}
}

TY  - CONF
ID  - 1418321
AU  - Mikušová, Zuzana - Pastucha, Matěj - Farka, Zdeněk - Čunderlová, Veronika - Skládal, Petr
PY  - 2018
TI  - Amperometric immunosensor for diagnosis of albuminuria
SN  - 9788021089419
KW  - Biosensor
KW  - Amperometry
KW  - Sandwich assay
KW  - Human serum albumin
KW  - Albuminuria
UR  - http://phdretreat.ceitec.cz/download/174
N2  - Human serum albumin (HSA) is a plasma protein involved in transportation of ligands to specific targets. Its increased presence in urine (microalbuminuria) is considered as an indication of diabetic nephropathy. Currently, HSA is being typically detected by immunoassays such as ELISA. These methods provide high sensitivity; however, they are time consuming, complicated and expensive. One of the possibilities to over-come these disadvantages is to use electrochemical biosensors that offer operational simplicity, short analysis times and miniaturization possibilities for point-of-care diagnosis. The electrochemical biosensors are typically based on screen-printed electrodes (SPEs) due to their low cost, portability and mass production capabilities. We have developed an amperometric immunosensor for rapid diagnostics of albumi-nuria. Anti-HSA antibody was immobilized to the surface of SPEs that allowed specific capture of HSA. The detection was based on binding of secondary antibody conjugated with horseradish peroxidase (HRP), that provides oxidation of 3,3´,5,5´-tetramethylbenzidine (TMB) in presence of H2O2. Alternatively, Prussian blue catalytic nanoparticles were employed as convenient enzyme replacement. SPEs based on gold and carbon (including carbon nanotubes) working electrode were compared. This method has proved to be suitable for determination of clinically relevant concentrations of HSA in urine, optimization focused on better detection limit is in progress.
ER  -

MIKUŠOVÁ, Zuzana, Matěj PASTUCHA, Zdeněk FARKA, Veronika ČUNDERLOVÁ and Petr SKLÁDAL. Amperometric immunosensor for diagnosis of albuminuria. In \textit{CEITEC PhD and Postdoc Retreat}. 2018. ISBN~978-80-210-8941-9.

Detailed Information on Publication Record