Amperometric immunosensor for diagnosis of albuminuria

MIKUŠOVÁ, Zuzana, Matěj PASTUCHA, Zdeněk FARKA, Veronika ČUNDERLOVÁ and Petr SKLÁDAL. Amperometric immunosensor for diagnosis of albuminuria. In CEITEC PhD and Postdoc Retreat. 2018. ISBN 978-80-210-8941-9.

Basic information

• Original name: Amperometric immunosensor for diagnosis of albuminuria
• Authors: MIKUŠOVÁ, Zuzana (703 Slovakia, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution), Veronika ČUNDERLOVÁ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: CEITEC PhD and Postdoc Retreat, 2018.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/18:00102777
• Organization unit: Central European Institute of Technology
• ISBN: 978-80-210-8941-9
• Keywords in English: Biosensor; Amperometry; Sandwich assay; Human serum albumin; Albuminuria
• Tags: rivok
• Tags: International impact

Abstract

Human serum albumin (HSA) is a plasma protein involved in transportation of ligands to specific targets. Its increased presence in urine (microalbuminuria) is considered as an indication of diabetic nephropathy. Currently, HSA is being typically detected by immunoassays such as ELISA. These methods provide high sensitivity; however, they are time consuming, complicated and expensive. One of the possibilities to over-come these disadvantages is to use electrochemical biosensors that offer operational simplicity, short analysis times and miniaturization possibilities for point-of-care diagnosis. The electrochemical biosensors are typically based on screen-printed electrodes (SPEs) due to their low cost, portability and mass production capabilities. We have developed an amperometric immunosensor for rapid diagnostics of albumi-nuria. Anti-HSA antibody was immobilized to the surface of SPEs that allowed specific capture of HSA. The detection was based on binding of secondary antibody conjugated with horseradish peroxidase (HRP), that provides oxidation of 3,3´,5,5´-tetramethylbenzidine (TMB) in presence of H2O2. Alternatively, Prussian blue catalytic nanoparticles were employed as convenient enzyme replacement. SPEs based on gold and carbon (including carbon nanotubes) working electrode were compared. This method has proved to be suitable for determination of clinically relevant concentrations of HSA in urine, optimization focused on better detection limit is in progress.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR