Intracellular and extracellular retinoid-like activity of
widespread cyanobacterial species

J 2018

Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species

PRIEBOJOVÁ, Jana, Klára HILSCHEROVÁ, Tereza PROCHÁZKOVÁ, Eliška SYCHROVÁ, Marie SMUTNÁ et. al.

Basic information

Original name

Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species

Authors

PRIEBOJOVÁ, Jana (703 Slovakia, belonging to the institution), Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution), Tereza PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Eliška SYCHROVÁ (203 Czech Republic, belonging to the institution) and Marie SMUTNÁ (203 Czech Republic, belonging to the institution)

Edition

Ecotoxicology and Environmental Safety, San Diego (CA) USA, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, 0147-6513

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.527

RIV identification code

RIV/00216224:14310/18:00100969

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ecoenv.2017.12.048

UT WoS

000423651600037

Keywords in English

Cyanobacteria; Retinoids; Retinoid-like activity; Extract; Exudates; ATRA

Abstract

V originále

Cyanobacterial species produce wide range of bioactive compounds. This study characterized production of retinoid-like compounds with embryotoxic and teratogenic potential by commonly occurring cyanobacterial species with tendency to form massive water blooms. The major goal was to simultaneously assess the intracellular and extracellular retinoid-like activity from several independent cultivations of one coccal (Microcystis aeruginosa) and four filamentous cyanobacteria (Aphanizomenon gracile, Cylindrospermopsis raciborskii, Linutothrix redekeii, and Planktothrix agardhii) and characterize the variability in its production among cultivations. The retinoid-like activity was evaluated by in vitro assay along with chemical analyses of nine retinoids: all-trans retinoic acid (ATRA), 9-cis retinoic acid (9cis-RA), 13cis-RA, 13cis-RA methyl ester, 5,6 epoxy RA, 4keto-ATRA, 4keto-retinal, 4hydoxy-retinoic add (4OH-ATRA), retinal and retinol. The production of retinoid-like compounds was recalculated per volume, per biomass dry weight and per cell to provide relevant data for risk assessment in relation to occurrence of massive water blooms in the environment. Total produced retinoid-like activity of five selected species ranged from 170 to 25,600 ng ATRA-equivalents (REQ)/g dm corresponding to 0.001-0.392 ng REQ/10(6) cyanobacterial cells. Results from chemical analyses showed that all tested extracts contained 4keto-ATRA and retinal. All-trans retinoic acid, 9/13cis-retinoic acid and 5,6 epoxyretinoic acid were detected in most exudate and extract samples. The reported results of recalculated total retinoid-like activity enable potential predictions of its production by the studied species in water blooms of known cell densities relevant for risk assessment.

Links

GP14-29370P, research and development project

Name: Běžné druhy sinic jako producenti teratogenních retinoidních látek

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PRIEBOJOVÁ, Jana, Klára HILSCHEROVÁ, Tereza PROCHÁZKOVÁ, Eliška SYCHROVÁ and Marie SMUTNÁ. Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species. Ecotoxicology and Environmental Safety. San Diego (CA) USA: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, vol. 150, April, p. 312-319. ISSN 0147-6513. Available from: https://dx.doi.org/10.1016/j.ecoenv.2017.12.048.

@article{1419178,
   author = {Priebojová, Jana and Hilscherová, Klára and Procházková, Tereza and Sychrová, Eliška and Smutná, Marie},
   article_location = {San Diego (CA) USA},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.ecoenv.2017.12.048},
   keywords = {Cyanobacteria; Retinoids; Retinoid-like activity; Extract; Exudates; ATRA},
   language = {eng},
   issn = {0147-6513},
   journal = {Ecotoxicology and Environmental Safety},
   title = {Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species},
   url = {https://www.sciencedirect.com/science/article/pii/S0147651317308825?via%3Dihub},
   volume = {150},
   year = {2018}
}

TY  - JOUR
ID  - 1419178
AU  - Priebojová, Jana - Hilscherová, Klára - Procházková, Tereza - Sychrová, Eliška - Smutná, Marie
PY  - 2018
TI  - Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species
JF  - Ecotoxicology and Environmental Safety
VL  - 150
IS  - April
SP  - 312-319
EP  - 312-319
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 01476513
KW  - Cyanobacteria
KW  - Retinoids
KW  - Retinoid-like activity
KW  - Extract
KW  - Exudates
KW  - ATRA
UR  - https://www.sciencedirect.com/science/article/pii/S0147651317308825?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0147651317308825?via%3Dihub
N2  - Cyanobacterial species produce wide range of bioactive compounds. This study characterized production of retinoid-like compounds with embryotoxic and teratogenic potential by commonly occurring cyanobacterial species with tendency to form massive water blooms. The major goal was to simultaneously assess the intracellular and extracellular retinoid-like activity from several independent cultivations of one coccal (Microcystis aeruginosa) and four filamentous cyanobacteria (Aphanizomenon gracile, Cylindrospermopsis raciborskii, Linutothrix redekeii, and Planktothrix agardhii) and characterize the variability in its production among cultivations. The retinoid-like activity was evaluated by in vitro assay along with chemical analyses of nine retinoids: all-trans retinoic acid (ATRA), 9-cis retinoic acid (9cis-RA), 13cis-RA, 13cis-RA methyl ester, 5,6 epoxy RA, 4keto-ATRA, 4keto-retinal, 4hydoxy-retinoic add (4OH-ATRA), retinal and retinol. The production of retinoid-like compounds was recalculated per volume, per biomass dry weight and per cell to provide relevant data for risk assessment in relation to occurrence of massive water blooms in the environment. Total produced retinoid-like activity of five selected species ranged from 170 to 25,600 ng ATRA-equivalents (REQ)/g dm corresponding to 0.001-0.392 ng REQ/10(6) cyanobacterial cells. Results from chemical analyses showed that all tested extracts contained 4keto-ATRA and retinal. All-trans retinoic acid, 9/13cis-retinoic acid and 5,6 epoxyretinoic acid were detected in most exudate and extract samples. The reported results of recalculated total retinoid-like activity enable potential predictions of its production by the studied species in water blooms of known cell densities relevant for risk assessment.
ER  -

PRIEBOJOVÁ, Jana, Klára HILSCHEROVÁ, Tereza PROCHÁZKOVÁ, Eliška SYCHROVÁ and Marie SMUTNÁ. Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species. \textit{Ecotoxicology and Environmental Safety}. San Diego (CA) USA: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, vol.~150, April, p.~312-319. ISSN~0147-6513. Available from: https://dx.doi.org/10.1016/j.ecoenv.2017.12.048.

Detailed Information on Publication Record