J 2018

Impact of acute and subchronic inhalation exposure to PbO nanoparticles on mice

LEBEDOVÁ, Jana, Zuzana NOVÁKOVÁ, Z. VECERA, marcela BUCHTOVÁ, Jana DUMKOVÁ et. al.

Basic information

Original name

Impact of acute and subchronic inhalation exposure to PbO nanoparticles on mice

Authors

LEBEDOVÁ, Jana (203 Czech Republic, belonging to the institution), Zuzana NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Z. VECERA (203 Czech Republic), marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution), Jana DUMKOVÁ (203 Czech Republic, belonging to the institution), B. DOCEKAL (203 Czech Republic), Lucie BLÁHOVÁ (203 Czech Republic, belonging to the institution), P. MIKUSKA (203 Czech Republic), I. MISEK (203 Czech Republic), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

NANOTOXICOLOGY, ABINGDON, TAYLOR & FRANCIS LTD, 2018, 1743-5390

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.955

RIV identification code

RIV/00216224:14310/18:00102887

Organization unit

Faculty of Science

DOI

UT WoS

000430425800002

Keywords in English

Nanoparticles; subchronic; lead; oxidative stress; accumulation; tissue damage

Tags

Tags

International impact, Reviewed
Změněno: 7/3/2019 10:54, Mgr. Radek Ševčík, Ph.D.

Abstract

V originále

Lead nanoparticles (NPs) are released into air from metal processing, road transport or combustion processes. Inhalation exposure is therefore very likely to occur. However, even though the effects of bulk lead are well known, there is limited knowledge regarding impact of Pb NPs inhalation. This study focused on acute and subchronic exposures to lead oxide nanoparticles (PbO NPs). Mice were exposed to PbO NPs in whole body inhalation chambers for 4-72h in acute experiment (4.05x10(6) PbO NPs/cm(3)), and for 1-11 weeks in subchronic experiment (3.83x10(5) particles/cm(3) in lower and 1.93x10(6) particles/cm(3) in higher exposure group). Presence of NPs was confirmed in all studied organs, including brain, which is very important considering lead neurotoxicity. Lead concentration gradually increased in all tissues depending on the exposure concentration and duration. The most burdened organs were lung and kidney, however liver and brain also showed significant increase of lead concentration during exposure. Histological analysis documented numerous morphological alterations and tissue damage, mainly in lung, but also in liver. Mild pathological changes were observed also in kidney and brain. Levels of glutathione (reduced and oxidized) were modulated mainly in lung in both, acute and subchronic exposures. Increase of lipid peroxidation was observed in kidney after acute exposure. This study characterized impacts of short to longer-term inhalation exposure, proved transport of PbO NPs to secondary organs, documented time and concentration dependent gradual increase of Pb concentration and histopathological damage in tissues.

Links

CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód MU
Name: RECETOX RI - OP VVV (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR, Priority axis 1: Strengthening capacities for high-quality research
LM2015051, research and development project
Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR