Heavy water enhancement of fluorescence signal in
reversed-phase liquid chromatography

J 2018

Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography

TÁBORSKÝ, Petr, Josef KUČERA, Jan JUŘICA and Ondřej PEŠ

Basic information

Original name

Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography

Authors

TÁBORSKÝ, Petr (203 Czech Republic, belonging to the institution), Josef KUČERA (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution) and Ondřej PEŠ (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, Amsterdam, Elsevier, 2018, 1570-0232

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.813

RIV identification code

RIV/00216224:14110/18:00102906

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.jchromb.2018.05.037

UT WoS

000439403100002

Keywords in English

Liquid chromatography; Heavy water; Fluorescence; Deuterium; Mass spectrometry; Doxorubicin; Fluorescein; Vitamin B2

Abstract

V originále

Liquid chromatography with fluorescence detection has been used in analyses demanding high sensitivity and selectivity. As majority systems rely on reversed-phase columns with water being the main component of the mobile phase, fluorescent compounds with emission maxima higher than 500 nm might be dynamically quenched. A simple replacement of H2O with D2O enhanced the sensitivity for selected compounds by 10–200 per cent. Affected compounds included an anti-cancer drug doxorubicin, a luminescent probe fluorescein, and naturally occurring forms of vitamin B2. Similar levels of enhancement were obtained by fluorescence spectrometry. Such simple yet effective approach may greatly improve HPLC analyses coupled to fluorescence detection.

Links

MUNI/A/0910/2017, interní kód MU

Name: Studium molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: stumopo)

Investor: Masaryk University, Category A

MUNI/A/1132/2017, interní kód MU

Name: Behaviorální psychofarmakologie a farmakokinetika v preklinickém výzkumu léčiv

Investor: Masaryk University, Category A

Citovat

TÁBORSKÝ, Petr, Josef KUČERA, Jan JUŘICA and Ondřej PEŠ. Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. Amsterdam: Elsevier, 2018, vol. 1092, AUG 15 2018, p. 7-14. ISSN 1570-0232. Available from: https://dx.doi.org/10.1016/j.jchromb.2018.05.037.

@article{1419710,
   author = {Táborský, Petr and Kučera, Josef and Juřica, Jan and Peš, Ondřej},
   article_location = {Amsterdam},
   article_number = {AUG 15 2018},
   doi = {http://dx.doi.org/10.1016/j.jchromb.2018.05.037},
   keywords = {Liquid chromatography; Heavy water; Fluorescence; Deuterium; Mass spectrometry; Doxorubicin; Fluorescein; Vitamin B2},
   language = {eng},
   issn = {1570-0232},
   journal = {JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES},
   title = {Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography},
   url = {https://www.sciencedirect.com/science/article/pii/S157002321830669X},
   volume = {1092},
   year = {2018}
}

TY  - JOUR
ID  - 1419710
AU  - Táborský, Petr - Kučera, Josef - Juřica, Jan - Peš, Ondřej
PY  - 2018
TI  - Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography
JF  - JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
VL  - 1092
IS  - AUG 15 2018
SP  - 7-14
EP  - 7-14
PB  - Elsevier
SN  - 15700232
KW  - Liquid chromatography
KW  - Heavy water
KW  - Fluorescence
KW  - Deuterium
KW  - Mass spectrometry
KW  - Doxorubicin
KW  - Fluorescein
KW  - Vitamin B2
UR  - https://www.sciencedirect.com/science/article/pii/S157002321830669X
N2  - Liquid chromatography with fluorescence detection has been used in analyses demanding high sensitivity and selectivity. As majority systems rely on reversed-phase columns with water being the main component of the mobile phase, fluorescent compounds with emission maxima higher than 500 nm might be dynamically quenched. A simple replacement of H2O with D2O enhanced the sensitivity for selected compounds by 10–200 per cent. Affected compounds included an anti-cancer drug doxorubicin, a luminescent probe fluorescein, and naturally occurring forms of vitamin B2. Similar levels of enhancement were obtained by fluorescence spectrometry. Such simple yet effective approach may greatly improve HPLC analyses coupled to fluorescence detection.
ER  -

TÁBORSKÝ, Petr, Josef KUČERA, Jan JUŘICA and Ondřej PEŠ. Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography. \textit{JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES}. Amsterdam: Elsevier, 2018, vol.~1092, AUG 15 2018, p.~7-14. ISSN~1570-0232. Available from: https://dx.doi.org/10.1016/j.jchromb.2018.05.037.

Detailed Information on Publication Record