"Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach

CELICHOWSKI, Piotr, Mariusz J. NAWROCKI, Marta DYSZKIEWICZ-KONWINSKA, Maurycy JANKOWSKI, Joanna BUDNA, Artur BRYJA, Wieslawa KRANC, Sylwia BORYS, Sandra KNAP, Sylwia CIESIOLKA, Michal JEŠETA, Karolina PIASECKA-STRYCZYNSKA, Ronza KHOZMI, Dorota BUKOWSKA, Pawel ANTOSIK, Klaus P. BRUSSOW, Malgorzata BRUSKA, Michal NOWICKI, Maciej ZABEL and Bartosz KEMPISTY. "Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach. Biomed Research International. New York: Hindawi Publishing Corporation, 2018, vol. 2018, No 2863068, p. 1-10. ISSN 2314-6133. Available from: https://dx.doi.org/10.1155/2018/2863068.

Basic information

Original name

"Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach

Authors

CELICHOWSKI, Piotr (616 Poland), Mariusz J. NAWROCKI (616 Poland), Marta DYSZKIEWICZ-KONWINSKA (616 Poland), Maurycy JANKOWSKI (616 Poland), Joanna BUDNA (616 Poland), Artur BRYJA (616 Poland), Wieslawa KRANC (616 Poland), Sylwia BORYS (616 Poland), Sandra KNAP (616 Poland), Sylwia CIESIOLKA (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Karolina PIASECKA-STRYCZYNSKA (616 Poland), Ronza KHOZMI (616 Poland), Dorota BUKOWSKA (616 Poland), Pawel ANTOSIK (616 Poland), Klaus P. BRUSSOW (616 Poland), Malgorzata BRUSKA (616 Poland), Michal NOWICKI (616 Poland), Maciej ZABEL (616 Poland) and Bartosz KEMPISTY (616 Poland, guarantor)

Edition

Biomed Research International, New York, Hindawi Publishing Corporation, 2018, 2314-6133

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30401 Health-related biotechnology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.197

RIV identification code

RIV/00216224:14110/18:00102953

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1155/2018/2863068

UT WoS

000419696500001

Keywords in English

RNA Metabolic Process

Tags

14110411, EL OK, rivok

Tags

International impact, Reviewed

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Abstract

V originále

The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in "oocytes RNA synthesis" in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/ proteins belonging to "positive regulation of RNA metabolic process" ontology group were investigated by STRING10 software. Using microarray assays, we found expression of 12258 porcine transcripts. Genes with fold change higher than |2| and with corrected.. value lower than 0.05 were considered as differentially expressed. The ontology group "positive regulation of RNA metabolic process" involved differential expression of AR, INHBA, WWTR1, FOS, MEF2C, VEGFA, IKZF2, IHH, RORA, MAP3K1, NFAT5, SMARCA1, EGR1, EGR2, MITF, SMAD4, APP, and NR5A1 transcripts. Since all of the presented genes were downregulated after IVM, we suggested that they might be significantly involved in regulation of RNA synthesis before reaching oocyte MII stage. Higher expression of "RNA metabolic process" related genes before IVM indicated that they might be recognized as important markers and specific "transcriptomic fingerprint" of RNA template accumulation and storage for further porcine embryos growth and development.