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@proceedings{1420072,
author = {Kostolanská, Katarína and Novotná, Lucie and Peš, Ondřej and Táborská, Eva},
booktitle = {16th Czech - Slovak Spectroscopic Conference},
keywords = {cortisol; hair; LC MS; surrogate; stress},
language = {eng},
isbn = {978-80-88195-06-1},
title = {Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte},
url = {http://16cssc2018.spektroskopie.cz/files/CSSC_2018_BOOK_OF_ABSTRACTS_FINAL.pdf},
year = {2018}
}
TY - CONF
ID - 1420072
AU - Kostolanská, Katarína - Novotná, Lucie - Peš, Ondřej - Táborská, Eva
PY - 2018
TI - Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte
SN - 9788088195061
KW - cortisol
KW - hair
KW - LC MS
KW - surrogate
KW - stress
UR - http://16cssc2018.spektroskopie.cz/files/CSSC_2018_BOOK_OF_ABSTRACTS_FINAL.pdf
N2 - Cortisol is a glucocorticoid hormone, which plays an important role in stressful situations when stressors activate the hypothalamic-pituitary-adrenocortical (HPA) system. Cortisol has been routinely analyzed in the laboratory from saliva, serum/plasma, and/or urine. However, the HPA axis represents a highly dynamic system influenced by several factors and expose circadian rhythmicity. When analyzed in hair, it can allow the unique possibility to assess the long-term systemic levels and obtain a retrospective index of long-term hormone secretion. Based on an average hair growth rate of 1 cm per month, it is possible to distribute the hair along the length of individual segments and determine the concentration of cortisol reflecting secretion in the monitored period. Online SPE LC-MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.
ER -
KOSTOLANSKÁ, Katarína, Lucie NOVOTNÁ, Ondřej PEŠ a Eva TÁBORSKÁ. Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte. In \textit{16th Czech - Slovak Spectroscopic Conference}. 2018. ISBN~978-80-88195-06-1.
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