Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte

KOSTOLANSKÁ, Katarína, Lucie NOVOTNÁ, Ondřej PEŠ and Eva TÁBORSKÁ. Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte. In 16th Czech - Slovak Spectroscopic Conference. 2018. ISBN 978-80-88195-06-1.

Basic information

• Original name: Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte
• Authors: KOSTOLANSKÁ, Katarína (703 Slovakia, belonging to the institution), Lucie NOVOTNÁ (203 Czech Republic, belonging to the institution), Ondřej PEŠ (203 Czech Republic, guarantor, belonging to the institution) and Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution).
• Edition: 16th Czech - Slovak Spectroscopic Conference, 2018.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10609 Biochemical research methods
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14110/18:00102960
• Organization unit: Faculty of Medicine
• ISBN: 978-80-88195-06-1
• Keywords in English: cortisol; hair; LC MS; surrogate; stress

Abstract

Cortisol is a glucocorticoid hormone, which plays an important role in stressful situations when stressors activate the hypothalamic-pituitary-adrenocortical (HPA) system. Cortisol has been routinely analyzed in the laboratory from saliva, serum/plasma, and/or urine. However, the HPA axis represents a highly dynamic system influenced by several factors and expose circadian rhythmicity. When analyzed in hair, it can allow the unique possibility to assess the long-term systemic levels and obtain a retrospective index of long-term hormone secretion. Based on an average hair growth rate of 1 cm per month, it is possible to distribute the hair along the length of individual segments and determine the concentration of cortisol reflecting secretion in the monitored period. Online SPE LC-MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.

Links

• MUNI/A/0910/2017, interní kód MU: Name: Studium molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: stumopo)

Investor: Masaryk University, Category A