| POROKH, Volodymyr, Petr VAŇHARA, Tomáš BÁRTA, Lucie JUREČKOVÁ, Dáša BOHAČIAKOVÁ, Veronika POSPÍŠILOVÁ, Daniela MINÁRIKOVÁ, Zuzana HOLUBCOVÁ, Vendula PELKOVÁ, Karel SOUCEK and Aleš HAMPL. Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells. Stem Cells and Development. NEW ROCHELLE: Mary Ann Liebert, Inc.., 2018, vol. 27, No 16, p. 1077-1084. ISSN 1547-3287. Available from: https://dx.doi.org/10.1089/scd.2018.0017. |
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@article{1420075,
author = {Porokh, Volodymyr and Vaňhara, Petr and Bárta, Tomáš and Jurečková, Lucie and Bohačiaková, Dáša and Pospíšilová, Veronika and Mináriková, Daniela and Holubcová, Zuzana and Pelková, Vendula and Soucek, Karel and Hampl, Aleš},
article_location = {NEW ROCHELLE},
article_number = {16},
doi = {http://dx.doi.org/10.1089/scd.2018.0017},
keywords = {embryonic stem cells; centrosomes; multipolar mitoses; Cripto-1; culture adaptation},
language = {eng},
issn = {1547-3287},
journal = {Stem Cells and Development},
title = {Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells},
url = {https://www.liebertpub.com/doi/10.1089/scd.2018.0017},
volume = {27},
year = {2018}
}
TY - JOUR
ID - 1420075
AU - Porokh, Volodymyr - Vaňhara, Petr - Bárta, Tomáš - Jurečková, Lucie - Bohačiaková, Dáša - Pospíšilová, Veronika - Mináriková, Daniela - Holubcová, Zuzana - Pelková, Vendula - Soucek, Karel - Hampl, Aleš
PY - 2018
TI - Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells
JF - Stem Cells and Development
VL - 27
IS - 16
SP - 1077-1084
EP - 1077-1084
PB - Mary Ann Liebert, Inc..
SN - 15473287
KW - embryonic stem cells
KW - centrosomes
KW - multipolar mitoses
KW - Cripto-1
KW - culture adaptation
UR - https://www.liebertpub.com/doi/10.1089/scd.2018.0017
N2 - Chromosomal instability evoked by abnormalities in centrosome numbers has been traditionally considered as a hallmark of aberrant, typically cancerous or senescent cells. We have reported previously that pristine human embryonic stem cells (hESC) suffer from high frequency of supernumerary centrosomes and hence may be prone to undergo abnormal mitotic divisions. We have also unraveled that this phenomenon of multicentrosomal mitoses vanishes with prolonged time in culture and with initiation of differentiation, and it is strongly affected by the culture substratum. In this study, we report for the first time that Cripto-1 protein (teratocarcinoma-derived growth factor 1, epidermal growth factor-Cripto/FRL-1/Cryptic) produced by hESC represents a factor capable of inducing formation of supernumerary centrosomes in cultured hESC. Elimination of Cripto-1 signaling on the other hand restores the normal number of centrosomes in hESC. Linking the secretory phenotype of hESC to the centrosomal metabolism may help to develop better strategies for propagation of stable and safe bioindustrial and clinical grade cultures of hESC. From a broader point of view, it may lead to unravelling Cripto-1 as a micro-environmental factor contributing to adverse cell behaviors in vivo.
ER -
POROKH, Volodymyr, Petr VAŇHARA, Tomáš BÁRTA, Lucie JUREČKOVÁ, Dáša BOHAČIAKOVÁ, Veronika POSPÍŠILOVÁ, Daniela MINÁRIKOVÁ, Zuzana HOLUBCOVÁ, Vendula PELKOVÁ, Karel SOUCEK and Aleš HAMPL. Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells. \textit{Stem Cells and Development}. NEW ROCHELLE: Mary Ann Liebert, Inc.., 2018, vol.~27, No~16, p.~1077-1084. ISSN~1547-3287. Available from: https://dx.doi.org/10.1089/scd.2018.0017.
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