a 2018

Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water

KUČERA, Josef, Ondřej PEŠ, Ivona VORÁČOVÁ and Petr TÁBORSKÝ

Basic information

Original name

Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water

Authors

KUČERA, Josef (203 Czech Republic, guarantor, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Ivona VORÁČOVÁ and Petr TÁBORSKÝ (203 Czech Republic, belonging to the institution)

Edition

16th Czech - Slovak Spectroscopic Conference 2018, 2018

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/18:00103114

Organization unit

Faculty of Science

ISBN

978-80-88195-06-1

Keywords in English

Fluorescence; enhancement; quenching of luminescence; deuterated water; anthracyclines; HPLC; chromatography; capillary electrophoresis; CE-LIF
Změněno: 25/6/2018 10:48, Mgr. Josef Kučera, Ph.D.

Abstract

V originále

Fluorescent molecules with emission maxima above 500 nm are often dynamically quenched by OH oscillators present in water molecules. We have changed H2O for D2O to obtain higher fluorescence intensity signal. Our experiments carried out on spectrofluorometer have proved that anthracyclines can achieve about 300 % higher response in D2O as solvent. The affect can be employed in various analytical methods including HPLC and capillary electrophoresis coupled to fluorescence detection.