Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water

KUČERA, Josef, Ondřej PEŠ, Ivona VORÁČOVÁ and Petr TÁBORSKÝ. Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water. In 16th Czech - Slovak Spectroscopic Conference 2018. 2018. ISBN 978-80-88195-06-1.

Basic information

• Original name: Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water
• Authors: KUČERA, Josef (203 Czech Republic, guarantor, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Ivona VORÁČOVÁ and Petr TÁBORSKÝ (203 Czech Republic, belonging to the institution).
• Edition: 16th Czech - Slovak Spectroscopic Conference 2018, 2018.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/18:00103114
• Organization unit: Faculty of Science
• ISBN: 978-80-88195-06-1
• Keywords in English: Fluorescence; enhancement; quenching of luminescence; deuterated water; anthracyclines; HPLC; chromatography; capillary electrophoresis; CE-LIF

Abstract

Fluorescent molecules with emission maxima above 500 nm are often dynamically quenched by OH oscillators present in water molecules. We have changed H2O for D2O to obtain higher fluorescence intensity signal. Our experiments carried out on spectrofluorometer have proved that anthracyclines can achieve about 300 % higher response in D2O as solvent. The affect can be employed in various analytical methods including HPLC and capillary electrophoresis coupled to fluorescence detection.