Detailed Information on Publication Record
2018
Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus
JELINKOVA, Pavlina, Radek VESELÝ, Krystina CIHALOVA, Dagmar HEGEROVA, Abd Alrahman Ali Ananbeh HANADI et. al.Basic information
Original name
Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus
Authors
JELINKOVA, Pavlina (203 Czech Republic), Radek VESELÝ (203 Czech Republic, belonging to the institution), Krystina CIHALOVA (203 Czech Republic), Dagmar HEGEROVA (203 Czech Republic), Abd Alrahman Ali Ananbeh HANADI (203 Czech Republic), Lukas RICHTERA (203 Czech Republic), Kristyna SMERKOVA (203 Czech Republic), Martin BRTNICKY (203 Czech Republic), Jindrich KYNICKY (203 Czech Republic), Amitava MOULICK (203 Czech Republic) and Vojtech ADAM (203 Czech Republic, guarantor)
Edition
Environmental Research, SAN DIEGO, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, 0013-9351
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30304 Public and environmental health
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.026
RIV identification code
RIV/00216224:14110/18:00103299
Organization unit
Faculty of Medicine
UT WoS
000445318200042
Keywords in English
Growth inhibition; Metals; Microbes; Oxidative stress; Antibiotic resistance
Tags
International impact, Reviewed
Změněno: 9/2/2019 22:27, Soňa Böhmová
Abstract
V originále
The presented study deals with the observation of properties of methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in the toxic arsenic environment and influence of arsenic on antioxidant capacity. Two forms of arsenic (As(III), As(V)) with different concentrations were used for induction of the oxidative stress in tested strains. Microbiological methods showed that the growth inhibition of MSSA was higher than that of MRSA in presence of both arsenic ions. As(III) showed 24% and 33% higher anti-microbial effects than As(V) against MSSA and MRSA respectively. A similar result was found also in the experiment of reduction of biofilm-formation. By using spectrophotometry, it was revealed that As(III) induced higher antioxidant production in both bacterial cultures. Methicillin-susceptible S. aureus produced an app. 50 mg equivalent of gallic acid (GAE/1 mg of protein) and MRSA produced an app. 15 mg of GAE/1 mg of protein. The productions of metallothionein in MSSA and MRSA were decreased up to 62.41% and 55.84% respectively in presence of As ions. Reduction of As(III) and As(V) concentrations leads to a decrease in antioxidant production and increased the formation of metallothionein. All of these changes in the results were found to be significant statistically. Taken together, these experiments proved that in comparison with MSSA, MRSA is less susceptible not only to the antimicrobial effects of antibiotics but also against effects caused by metalloids, as arsenic. Thus, it can be stated that MRSA abounds with complex defensive mechanisms, which may in the future constitute significant problem in the efficiency of antibiotics alternatives as metal ions or nanoparticles.