Effect of arsenic (III and V) on oxidative stress parameters in
resistant and susceptible Staphylococcus aureus

J 2018

Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus

JELINKOVA, Pavlina, Radek VESELÝ, Krystina CIHALOVA, Dagmar HEGEROVA, Abd Alrahman Ali Ananbeh HANADI et. al.

Basic information

Original name

Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus

Authors

JELINKOVA, Pavlina (203 Czech Republic), Radek VESELÝ (203 Czech Republic, belonging to the institution), Krystina CIHALOVA (203 Czech Republic), Dagmar HEGEROVA (203 Czech Republic), Abd Alrahman Ali Ananbeh HANADI (203 Czech Republic), Lukas RICHTERA (203 Czech Republic), Kristyna SMERKOVA (203 Czech Republic), Martin BRTNICKY (203 Czech Republic), Jindrich KYNICKY (203 Czech Republic), Amitava MOULICK (203 Czech Republic) and Vojtech ADAM (203 Czech Republic, guarantor)

Edition

Environmental Research, SAN DIEGO, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, 0013-9351

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.026

RIV identification code

RIV/00216224:14110/18:00103299

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.envres.2018.06.024

UT WoS

000445318200042

Keywords in English

Growth inhibition; Metals; Microbes; Oxidative stress; Antibiotic resistance

Abstract

V originále

The presented study deals with the observation of properties of methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in the toxic arsenic environment and influence of arsenic on antioxidant capacity. Two forms of arsenic (As(III), As(V)) with different concentrations were used for induction of the oxidative stress in tested strains. Microbiological methods showed that the growth inhibition of MSSA was higher than that of MRSA in presence of both arsenic ions. As(III) showed 24% and 33% higher anti-microbial effects than As(V) against MSSA and MRSA respectively. A similar result was found also in the experiment of reduction of biofilm-formation. By using spectrophotometry, it was revealed that As(III) induced higher antioxidant production in both bacterial cultures. Methicillin-susceptible S. aureus produced an app. 50 mg equivalent of gallic acid (GAE/1 mg of protein) and MRSA produced an app. 15 mg of GAE/1 mg of protein. The productions of metallothionein in MSSA and MRSA were decreased up to 62.41% and 55.84% respectively in presence of As ions. Reduction of As(III) and As(V) concentrations leads to a decrease in antioxidant production and increased the formation of metallothionein. All of these changes in the results were found to be significant statistically. Taken together, these experiments proved that in comparison with MSSA, MRSA is less susceptible not only to the antimicrobial effects of antibiotics but also against effects caused by metalloids, as arsenic. Thus, it can be stated that MRSA abounds with complex defensive mechanisms, which may in the future constitute significant problem in the efficiency of antibiotics alternatives as metal ions or nanoparticles.

Citovat

JELINKOVA, Pavlina, Radek VESELÝ, Krystina CIHALOVA, Dagmar HEGEROVA, Abd Alrahman Ali Ananbeh HANADI, Lukas RICHTERA, Kristyna SMERKOVA, Martin BRTNICKY, Jindrich KYNICKY, Amitava MOULICK and Vojtech ADAM. Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus. Environmental Research. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, vol. 166, No 8, p. 394-401. ISSN 0013-9351. Available from: https://dx.doi.org/10.1016/j.envres.2018.06.024.

@article{1423839,
   author = {Jelinkova, Pavlina and Veselý, Radek and Cihalova, Krystina and Hegerova, Dagmar and Hanadi, Abd Alrahman Ali Ananbeh and Richtera, Lukas and Smerkova, Kristyna and Brtnicky, Martin and Kynicky, Jindrich and Moulick, Amitava and Adam, Vojtech},
   article_location = {SAN DIEGO},
   article_number = {8},
   doi = {http://dx.doi.org/10.1016/j.envres.2018.06.024},
   keywords = {Growth inhibition; Metals; Microbes; Oxidative stress; Antibiotic resistance},
   language = {eng},
   issn = {0013-9351},
   journal = {Environmental Research},
   title = {Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus},
   url = {http://www.elsevier.com},
   volume = {166},
   year = {2018}
}

TY  - JOUR
ID  - 1423839
AU  - Jelinkova, Pavlina - Veselý, Radek - Cihalova, Krystina - Hegerova, Dagmar - Hanadi, Abd Alrahman Ali Ananbeh - Richtera, Lukas - Smerkova, Kristyna - Brtnicky, Martin - Kynicky, Jindrich - Moulick, Amitava - Adam, Vojtech
PY  - 2018
TI  - Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus
JF  - Environmental Research
VL  - 166
IS  - 8
SP  - 394-401
EP  - 394-401
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 00139351
KW  - Growth inhibition
KW  - Metals
KW  - Microbes
KW  - Oxidative stress
KW  - Antibiotic resistance
UR  - http://www.elsevier.com
N2  - The presented study deals with the observation of properties of methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in the toxic arsenic environment and influence of arsenic on antioxidant capacity. Two forms of arsenic (As(III), As(V)) with different concentrations were used for induction of the oxidative stress in tested strains. Microbiological methods showed that the growth inhibition of MSSA was higher than that of MRSA in presence of both arsenic ions. As(III) showed 24% and 33% higher anti-microbial effects than As(V) against MSSA and MRSA respectively. A similar result was found also in the experiment of reduction of biofilm-formation. By using spectrophotometry, it was revealed that As(III) induced higher antioxidant production in both bacterial cultures. Methicillin-susceptible S. aureus produced an app. 50 mg equivalent of gallic acid (GAE/1 mg of protein) and MRSA produced an app. 15 mg of GAE/1 mg of protein. The productions of metallothionein in MSSA and MRSA were decreased up to 62.41% and 55.84% respectively in presence of As ions. Reduction of As(III) and As(V) concentrations leads to a decrease in antioxidant production and increased the formation of metallothionein. All of these changes in the results were found to be significant statistically. Taken together, these experiments proved that in comparison with MSSA, MRSA is less susceptible not only to the antimicrobial effects of antibiotics but also against effects caused by metalloids, as arsenic. Thus, it can be stated that MRSA abounds with complex defensive mechanisms, which may in the future constitute significant problem in the efficiency of antibiotics alternatives as metal ions or nanoparticles.
ER  -

JELINKOVA, Pavlina, Radek VESELÝ, Krystina CIHALOVA, Dagmar HEGEROVA, Abd Alrahman Ali Ananbeh HANADI, Lukas RICHTERA, Kristyna SMERKOVA, Martin BRTNICKY, Jindrich KYNICKY, Amitava MOULICK and Vojtech ADAM. Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus. \textit{Environmental Research}. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, vol.~166, No~8, p.~394-401. ISSN~0013-9351. Available from: https://dx.doi.org/10.1016/j.envres.2018.06.024.

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