AFM Monitoring the Influence o f Selected Cryoprotectants on Regeneration of Cryopreserved Cells ...

@article{1424436,
   author = {Golan, Martin and Jelínková, Šárka and Kratochvílová, Irena and Skládal, Petr and Pešl, Martin and Rotrekl, Vladimír and Přibyl, Jan},
   article_location = {Lausanne},
   article_number = {804},
   doi = {http://dx.doi.org/10.3389/fphys.2018.00804},
   keywords = {cryopreservation; cell stiffness; AFM; fluorescence microscopy; DMSO; PEG},
   language = {eng},
   issn = {1664-042X},
   journal = {Frontiers in Physiology},
   title = {AFM Monitoring the Influence o f Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties},
   url = {https://www.frontiersin.org/articles/10.3389/fphys.2018.00804/full},
   volume = {9},
   year = {2018}
}

TY  - JOUR
ID  - 1424436
AU  - Golan, Martin - Jelínková, Šárka - Kratochvílová, Irena - Skládal, Petr - Pešl, Martin - Rotrekl, Vladimír - Přibyl, Jan
PY  - 2018
TI  - AFM Monitoring the Influence o f Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties
JF  - Frontiers in Physiology
VL  - 9
IS  - 804
SP  - 1-10
EP  - 1-10
PB  - Frontiers Media
SN  - 1664042X
KW  - cryopreservation
KW  - cell stiffness
KW  - AFM
KW  - fluorescence microscopy
KW  - DMSO
KW  - PEG
UR  - https://www.frontiersin.org/articles/10.3389/fphys.2018.00804/full
N2  - Cryopreservation of cells (mouse embryonic fibroblasts) is a fundamental task for wide range of applications. In practice, cells are protected against damage during freezing by applications of specific cryoprotectants and freezing/melting protocols. In this study by using AFM and fluorescence microscopy we showed how selected cryoprotectants (dimethyl sulfoxide and polyethylene glycol) affected the cryopreserved cells mechanical properties (stiffness) and how these parameters are correlated with cytoskeleton damage and reconstruction. We showed how cryopreserved (frozen and thawed) cells' stiffness change according to type of applied cryoprotectant and its functionality in extracellular or intracellular space. We showed that AFM can be used as technique for investigation of cryopreserved cells surfaces state and development ex vivo. Our results offer a new perspective on the monitoring and characterization of frozen cells recovery by measuring changes in elastic properties by nanoindentation technique. This may lead to a new and detailed way of investigating the post-thaw development of cryopreserved cells which allows to distinguish between different cell parts.
ER  - 

GOLAN, Martin, Šárka JELÍNKOVÁ, Irena KRATOCHVÍLOVÁ, Petr SKLÁDAL, Martin PEŠL, Vladimír ROTREKL and Jan PŘIBYL. AFM Monitoring the Influence o f Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties. \textit{Frontiers in Physiology}. Lausanne: Frontiers Media, 2018, vol.~9, No~804, p.~1-10. ISSN~1664-042X. Available from: https://dx.doi.org/10.3389/fphys.2018.00804.