Molecular detection of Toxoplasma gondii in feathered game
intended for human consumption in the Czech Republic

J 2018

Molecular detection of Toxoplasma gondii in feathered game intended for human consumption in the Czech Republic

ŠKORPÍKOVÁ, Lucie, Nikol RESLOVÁ, Alena LORENCOVÁ, Radim PLHAL, Jakub DRIMAJ et. al.

Basic information

Original name

Molecular detection of Toxoplasma gondii in feathered game intended for human consumption in the Czech Republic

Name in Czech

Molekulární detekce Toxoplasma gondii u pernaté zvěře určené k lidské spotřebě v České republice

Authors

ŠKORPÍKOVÁ, Lucie (203 Czech Republic, guarantor, belonging to the institution), Nikol RESLOVÁ (203 Czech Republic, belonging to the institution), Alena LORENCOVÁ (203 Czech Republic), Radim PLHAL, Jakub DRIMAJ, Jiří KAMLER (203 Czech Republic) and Michal SLANÝ (203 Czech Republic)

Edition

International Journal of Food Microbiology, Elsevier Science, 2018, 0168-1605

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.006

RIV identification code

RIV/00216224:14310/18:00103356

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ijfoodmicro.2018.07.019

UT WoS

000449140800009

Keywords (in Czech)

Toxoplasmóza; Bezpečnost potravin; Real-time PCR; Genotypizace; Kachny; Bažanti

Keywords in English

Toxoplasmosis; Food safety; Real-time PCR; Genotyping; Ducks; Pheasants

Abstract

V originále

Toxoplasma gondii is an important ubiquitous protozoan parasite, which can infect almost all warm-blooded vertebrates, including humans. The diagnosis of T. gondii infection is crucial for the prevention, surveillance, and control of its transmission. Here, a triplex real-time PCR assay targeting the B1 gene and 529rep element was used to determine the presence of T. gondii in feathered game (Anas platyrhynchos and Phasianus colchicus) hunted in the Czech Republic. The prevalence of T. gondii was 5.4% in wild ducks (n = 280) and 3.4% in common pheasants (n = 350). Additionally, genotyping of 28 T. gondii-positive samples revealed the presence of archetypal genotypes II and III as well as non-archetypal genotypes combining both type II and III alleles. Our results suggest that consumption of feathered game could pose a risk of T. gondii transmission to humans in the Czech Republic.

Citovat

ŠKORPÍKOVÁ, Lucie, Nikol RESLOVÁ, Alena LORENCOVÁ, Radim PLHAL, Jakub DRIMAJ, Jiří KAMLER and Michal SLANÝ. Molecular detection of Toxoplasma gondii in feathered game intended for human consumption in the Czech Republic. International Journal of Food Microbiology. Elsevier Science, 2018, vol. 286, DEC, p. 75-79. ISSN 0168-1605. Available from: https://dx.doi.org/10.1016/j.ijfoodmicro.2018.07.019.

@article{1425336,
   author = {Škorpíková, Lucie and Reslová, Nikol and Lorencová, Alena and Plhal, Radim and Drimaj, Jakub and Kamler, Jiří and Slaný, Michal},
   article_number = {DEC},
   doi = {http://dx.doi.org/10.1016/j.ijfoodmicro.2018.07.019},
   keywords = {Toxoplasmosis; Food safety; Real-time PCR; Genotyping; Ducks; Pheasants},
   language = {eng},
   issn = {0168-1605},
   journal = {International Journal of Food Microbiology},
   title = {Molecular detection of Toxoplasma gondii in feathered game intended for human consumption in the Czech Republic},
   url = {https://www.sciencedirect.com/science/article/pii/S0168160518303763?via%3Dihub},
   volume = {286},
   year = {2018}
}

TY  - JOUR
ID  - 1425336
AU  - Škorpíková, Lucie - Reslová, Nikol - Lorencová, Alena - Plhal, Radim - Drimaj, Jakub - Kamler, Jiří - Slaný, Michal
PY  - 2018
TI  - Molecular detection of Toxoplasma gondii in feathered game intended for human consumption in the Czech Republic
JF  - International Journal of Food Microbiology
VL  - 286
IS  - DEC
SP  - 75-79
EP  - 75-79
PB  - Elsevier Science
SN  - 01681605
KW  - Toxoplasmosis
KW  - Food safety
KW  - Real-time PCR
KW  - Genotyping
KW  - Ducks
KW  - Pheasants
UR  - https://www.sciencedirect.com/science/article/pii/S0168160518303763?via%3Dihub
N2  - Toxoplasma gondii is an important ubiquitous protozoan parasite, which can infect almost all warm-blooded vertebrates, including humans. The diagnosis of T. gondii infection is crucial for the prevention, surveillance, and control of its transmission. Here, a triplex real-time PCR assay targeting the B1 gene and 529rep element was used to determine the presence of T. gondii in feathered game (Anas platyrhynchos and Phasianus colchicus) hunted in the Czech Republic. The prevalence of T. gondii was 5.4% in wild ducks (n = 280) and 3.4% in common pheasants (n = 350). Additionally, genotyping of 28 T. gondii-positive samples revealed the presence of archetypal genotypes II and III as well as non-archetypal genotypes combining both type II and III alleles. Our results suggest that consumption of feathered game could pose a risk of T. gondii transmission to humans in the Czech Republic.
ER  -

ŠKORPÍKOVÁ, Lucie, Nikol RESLOVÁ, Alena LORENCOVÁ, Radim PLHAL, Jakub DRIMAJ, Jiří KAMLER and Michal SLANÝ. Molecular detection of Toxoplasma gondii in feathered game intended for human consumption in the Czech Republic. \textit{International Journal of Food Microbiology}. Elsevier Science, 2018, vol.~286, DEC, p.~75-79. ISSN~0168-1605. Available from: https://dx.doi.org/10.1016/j.ijfoodmicro.2018.07.019.

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