Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum

ROUDNICKÝ, Pavel, Tomáš LOJA, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR and Martin KAŠNÝ. Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum. In 24th Helminthological Days. 2018. ISBN 978-80-7444-057-1.

Basic information

• Original name: Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum
• Authors: ROUDNICKÝ, Pavel (203 Czech Republic, guarantor, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution).
• Edition: 24th Helminthological Days, 2018.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: web konference
• RIV identification code: RIV/00216224:14310/18:00101088
• Organization unit: Faculty of Science
• ISBN: 978-80-7444-057-1
• Keywords in English: Monogenea; parasite; platyhelminth; laser microdissection; mass spectrometry; protein

Abstract

Laser microdissection is a combined microscopic-separation technique enabling to extract the samples of interest from histological slides - single cells, organs and tissues. The isolated samples can be further analysed and various parameters determined. The adoption of mass spectrometry analysis could give us the detail information about the sample composition and relative quantity of particular components (e.g. protein molecules) can be calculated too. Moreover, this technology enables the fast determination of distribution of selected molecule within the studied biological object. Thus, we can avoid the time-consuming preparation of specific antibodies in laboratory animals, which are widely used for protein tissue localization. In our current research, we used Eudiplozoon nipponicum, ectoparasitic blood-feeder from gills of Cyprinus carpio with aim to reveal the protein composition of its tegument region, gut and inner parenchyma. So far, we have identified 405 unique proteins in inner parenchyma/gut and 131 unique proteins in tegument. Among them, some already described functional molecules have been found.

Links

• GBP505/12/G112, research and development project: Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

• MUNI/A/0816/2017, interní kód MU: Name: Výzkum ekologicko-evolučních vztahů bezobratlých živočichů (Acronym: EKOLINKS)

Investor: Masaryk University, Category A