Laser microdissection and mass spectrometry: protein analysis
of strictly selected tissue from Eudiplozoon nipponicum

k 2018

Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum

ROUDNICKÝ, Pavel, Tomáš LOJA, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR et. al.

Basic information

Original name

Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum

Authors

ROUDNICKÝ, Pavel (203 Czech Republic, guarantor, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

24th Helminthological Days, 2018

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

web konference

RIV identification code

RIV/00216224:14310/18:00101088

Organization unit

Faculty of Science

ISBN

978-80-7444-057-1

Keywords in English

Monogenea; parasite; platyhelminth; laser microdissection; mass spectrometry; protein

Změněno: 31/7/2018 13:44, Mgr. Pavel Roudnický, Ph.D.

Abstract

V originále

Laser microdissection is a combined microscopic-separation technique enabling to extract the samples of interest from histological slides - single cells, organs and tissues. The isolated samples can be further analysed and various parameters determined. The adoption of mass spectrometry analysis could give us the detail information about the sample composition and relative quantity of particular components (e.g. protein molecules) can be calculated too. Moreover, this technology enables the fast determination of distribution of selected molecule within the studied biological object. Thus, we can avoid the time-consuming preparation of specific antibodies in laboratory animals, which are widely used for protein tissue localization. In our current research, we used Eudiplozoon nipponicum, ectoparasitic blood-feeder from gills of Cyprinus carpio with aim to reveal the protein composition of its tegument region, gut and inner parenchyma. So far, we have identified 405 unique proteins in inner parenchyma/gut and 131 unique proteins in tegument. Among them, some already described functional molecules have been found.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

MUNI/A/0816/2017, interní kód MU

Name: Výzkum ekologicko-evolučních vztahů bezobratlých živočichů (Acronym: EKOLINKS)

Investor: Masaryk University, Category A

Citovat

ROUDNICKÝ, Pavel, Tomáš LOJA, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR and Martin KAŠNÝ. Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum. In 24th Helminthological Days. 2018. ISBN 978-80-7444-057-1.

@proceedings{1425516,
   author = {Roudnický, Pavel and Loja, Tomáš and Potěšil, David and Zdráhal, Zbyněk and Gelnar, Milan and Kašný, Martin},
   booktitle = {24th Helminthological Days},
   keywords = {Monogenea; parasite; platyhelminth; laser microdissection; mass spectrometry; protein},
   language = {eng},
   isbn = {978-80-7444-057-1},
   title = {Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum},
   url = {https://hd2018.natur.cuni.cz/},
   year = {2018}
}

TY  - CONF
ID  - 1425516
AU  - Roudnický, Pavel - Loja, Tomáš - Potěšil, David - Zdráhal, Zbyněk - Gelnar, Milan - Kašný, Martin
PY  - 2018
TI  - Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum
SN  - 9788074440571
KW  - Monogenea
KW  - parasite
KW  - platyhelminth
KW  - laser microdissection
KW  - mass spectrometry
KW  - protein
UR  - https://hd2018.natur.cuni.cz/
L2  - https://hd2018.natur.cuni.cz/
N2  - Laser microdissection is a combined microscopic-separation technique enabling to extract the samples of interest from histological slides - single cells, organs and tissues. The isolated samples can be further analysed and various parameters determined. The adoption of mass spectrometry analysis could give us the detail information about the sample composition and relative quantity of particular components (e.g. protein molecules) can be calculated too. Moreover, this technology enables the fast determination of distribution of selected molecule within the studied biological object. Thus, we can avoid the time-consuming preparation of specific antibodies in laboratory animals, which are widely used for protein tissue localization. In our current research, we used Eudiplozoon nipponicum, ectoparasitic blood-feeder from gills of Cyprinus carpio with aim to reveal the protein composition of its tegument region, gut and inner parenchyma. So far, we have identified 405 unique proteins in inner parenchyma/gut and 131 unique proteins in tegument. Among them, some already described functional molecules have been found.
ER  -

ROUDNICKÝ, Pavel, Tomáš LOJA, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR and Martin KAŠNÝ. Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum. In \textit{24th Helminthological Days}. 2018. ISBN~978-80-7444-057-1.

Detailed Information on Publication Record