Detailed Information on Publication Record
2018
RS20L protein from bacterium Ralstonia solanacearum : Studies of lectin activity and oligomerization
KYSEĽ, Peter, Nikola KOSTLÁNOVÁ, Ondřej ŠULÁK, Eva FUJDIAROVÁ, Jan KOMÁREK et. al.Basic information
Original name
RS20L protein from bacterium Ralstonia solanacearum : Studies of lectin activity and oligomerization
Authors
KYSEĽ, Peter (703 Slovakia, belonging to the institution), Nikola KOSTLÁNOVÁ (203 Czech Republic, belonging to the institution), Ondřej ŠULÁK (203 Czech Republic, belonging to the institution), Eva FUJDIAROVÁ (203 Czech Republic, belonging to the institution), Jan KOMÁREK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Structural Glycoscience Summer School, 2018
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
France
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14740/18:00103381
Organization unit
Central European Institute of Technology
Keywords in English
Ralstonia; MBP; lectin; trimer; protein purification; analytical ultracentrifugation; isothermal titration calorimetry; protein crystallization; SDS-PAGE; glycan array; macroseeding
Tags
Změněno: 26/3/2019 15:20, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Protein RS20L from Ralstonia solanacearum was examined with structural and functional techniques. Oligomerization state and stability were tested by analytical ultracentrifugation. Isothermal titration calorimetry confirmed interaction with mannosylated oligodendrimers. Glycan array displayed binding to yeast mannan. The protein crystallization conditions were optimized.
Links
| LM2015043, research and development project |
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| LQ1601, research and development project |
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