| POHANKA, Miroslav, Jitka ZAKOVA and Ivo SEDLÁČEK. Digital camera-based lipase biosensor for the determination of paraoxon. SENSORS AND ACTUATORS B: CHEMICAL. Elsevier Science, 2018, vol. 273, November 2018, p. 610-615. ISSN 0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2018.06.084. |
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@article{1426988,
author = {Pohanka, Miroslav and Zakova, Jitka and Sedláček, Ivo},
article_number = {November 2018},
doi = {http://dx.doi.org/10.1016/j.snb.2018.06.084},
keywords = {Biosensor; Liopase; Fatty acid; Inhibition},
language = {eng},
issn = {0925-4005},
journal = {SENSORS AND ACTUATORS B: CHEMICAL},
title = {Digital camera-based lipase biosensor for the determination of paraoxon},
url = {http://dx.doi.org/10.1016/j.snb.2018.06.084},
volume = {273},
year = {2018}
}
TY - JOUR
ID - 1426988
AU - Pohanka, Miroslav - Zakova, Jitka - Sedláček, Ivo
PY - 2018
TI - Digital camera-based lipase biosensor for the determination of paraoxon
JF - SENSORS AND ACTUATORS B: CHEMICAL
VL - 273
IS - November 2018
SP - 610-615
EP - 610-615
PB - Elsevier Science
SN - 09254005
KW - Biosensor
KW - Liopase
KW - Fatty acid
KW - Inhibition
UR - http://dx.doi.org/10.1016/j.snb.2018.06.084
N2 - This work is focused on construction of a biosensor containing unique bacterial homogenate with high lipase activity and colorimetric type of assay where camera of a smartphone was chosen as a detector. The biosensor was constructed as a tool of lipase inhibitors and paraoxon served as a representative analyte inhibiting the lipase. Psychrophilic strains of bacteria isolated from in Antarctica were tested and the best isolate P4368 having huge lipase activity was chosen. Tween assay was performed as a standard method for lipase activity determination and indoxylacetate served as a substrate of lipase measurable by smartphone camera and R, G and B color channels digital analysis. Bacteria were homogenized and immobilized on polyvinylidene difluoride membrane and indoxylacetate was immobilized in the proximity of the homogenate. Paraoxon ethyl was analyzed by the standard method and by the camera-based biosensor. The biosensor-based assay was found to determine paraoxon with limit of detection 3.72×10 -8exp mol/l and IC50 value for paraoxon was 4.00×10 -6exp mol/l. A volume 5 microliter of sample was sufficient for the assay and the sample was applied directly without any processing. In a conclusion, a simple colorimetric biosensor for the determination of venomous compounds like organophosphorus pesticides and nerve agents was constructed and promising analytical parameters were received during its characterization.
ER -
POHANKA, Miroslav, Jitka ZAKOVA and Ivo SEDLÁČEK. Digital camera-based lipase biosensor for the determination of paraoxon. \textit{SENSORS AND ACTUATORS B: CHEMICAL}. Elsevier Science, 2018, vol.~273, November 2018, p.~610-615. ISSN~0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2018.06.084.
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