J 2018

Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

BURSOVA, S., L. NECIDOVA and Danka HARUŠTIAKOVÁ

Basic information

Original name

Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

Authors

BURSOVA, S. (203 Czech Republic), L. NECIDOVA (203 Czech Republic) and Danka HARUŠTIAKOVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

Food Control, Oxford, Elsevier Science Ltd, 2018, 0956-7135

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

21101 Food and beverages

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.248

RIV identification code

RIV/00216224:14310/18:00103467

Organization unit

Faculty of Science

DOI

UT WoS

000440390200041

Keywords in English

Baranyi-Roberts model; Enterotoxins; Cereulide; lmmunochromatography

Tags

Tags

International impact, Reviewed
Změněno: 23/4/2024 12:24, Mgr. Michal Petr

Abstract

V originále

Bacillus cereus may cause two different types of foodborne illness: diarrheal and emetic syndromes. Dried infant formulae are at risk of contamination by the B. cereus, which is reflected by the requirement for the microbiological examination of foods listed in Regulation (EC) 2073/2005, as amended. This study evaluates the growth and toxin production potential of B. cereus in reconstituted dried infant milk formula. Powdered milk was inoculated with approximately 1 log and 3 log cfu g(-1) of spore suspension of toxigenic B. cereus strains and then stored as reconstituted milk for 48 h at 8 degrees C, 15 degrees C, and 24 degrees C. The growth of B. cereus, tested by the plate method, and production of the emetic toxin and enterotoxins, tested by the gold-labelled immunosorbent assay, were regularly detected during 48 h of storage. The Baranyi-Roberts model was used to study the growth potential of B. cereus strains. In reconstituted infant milk, B. cereus did not grow at 8 degrees C during 48 h of storage. B. cereus multiplied and formed toxins only at 15 degrees C and 24 degrees C. The growth curve of the bacteria stored at 15 degrees C included the lag and exponential phases, and the growth was not completed during the period. The growth of B. cereus at 24 degrees C was completed, peaking within 27 and 20 h, with the low and high inoculum sizes, respectively. Detectable amounts of toxins were formed during the bacterial growth of around 5 log cfu ml(-1) (non-hemolytic enterotoxin) and 7 log cfu ml(-1) (hemolysin BL and emetic toxin). Model experiments evaluated consumer risk from B. cereus toxins resulting from extended storage of reconstituted infant milk formula at improper temperatures. (C) 2017 Elsevier Ltd. All rights reserved.