Detailed Information on Publication Record
2017
Immunomodulatory function of cysteine peptidase inhibitor from Eudiplozoon nipponicum
ILGOVÁ, Jana, Pavel ROUDNICKÝ, Jiří VOREL, Libor VOJTEK, Pavel HYRŠL et. al.Basic information
Original name
Immunomodulatory function of cysteine peptidase inhibitor from Eudiplozoon nipponicum
Authors
ILGOVÁ, Jana (703 Slovakia, guarantor, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Jiří VOREL (203 Czech Republic, belonging to the institution), Libor VOJTEK (203 Czech Republic), Pavel HYRŠL (203 Czech Republic, belonging to the institution), Jiří SALÁT (203 Czech Republic), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)
Edition
6th Workshop of the European Centre of IchthyoParasitology, 2017
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10613 Zoology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/17:00101129
Organization unit
Faculty of Science
Keywords in English
cystatin; inhibition; Eudiplozoon nipponicum; blood-feeding; immunomodulation; inflammation; cytokines
Změněno: 4/9/2018 10:40, Mgr. et Mgr. Jana Ilgová, Ph.D.
Abstract
V originále
Eudiplozoon nipponicum is a sanguivorous monogenean of East Asian origin which parasitizes the gills of the common carp, Cyprinus carpio. One of the mechanisms which enable successful parasitic lifestyle is the utilization of compounds contained in excretory-secretory products of the parasite for possible modulation of the host physiological processes. We identified cysteine peptidase inhibitor (type I cystatin, also known as stefin) among the molecules secreted by E. nipponicum. These inhibitory molecules are generally known as regulators of endogenous processes related to the protein degradation. Besides, they can affect the blood digestion, immunomodulation of the host and the repair of its tissue damaged by the parasite. Our study aims to reveal the immunomodulatory potential of the stefin of E. nipponicum. During in vitro assays we used recombinant stefin which is capable of inhibiting cathepsin B and L as well as legumain. Using qPCR method we characterized the changes in the expression of cytokines by immune cells stimulated by LPS. Recombinant stefin of E. nipponicum caused significant downregulation of selected cytokines (IL-1beta, IL-8, TNF-alpha, IL-6 and IL-10) by porcine alveolar macrophages and monocyte derived macrophages. Our finding indicates possible role of this inhibitor in immunomodulation of the host, i.e., the infiltration of neutrophils, suppression of inflammatory response and differentiation of T cells. Luminol-enhanced chemiluminescence assays did not prove any significant effect of stefin on the activation of complement in carp’s plasma or oxidative burst in full blood.
Links
| GBP505/12/G112, research and development project |
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| MUNI/A/0816/2017, interní kód MU |
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