k 2018

Monogenea, fish parasites and their unknown molecules

VOREL, Jiří, Marie JANKŮJOVÁ, Jan OPPELT, Filip PARDY, David POTĚŠIL et. al.

Basic information

Original name

Monogenea, fish parasites and their unknown molecules

Authors

VOREL, Jiří (203 Czech Republic, guarantor, belonging to the institution), Marie JANKŮJOVÁ (203 Czech Republic), Jan OPPELT (203 Czech Republic), Filip PARDY (203 Czech Republic), David POTĚŠIL (203 Czech Republic), Pavel ROUDNICKÝ (203 Czech Republic), Jana ILGOVÁ (703 Slovakia), Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Libor MIKEŠ (203 Czech Republic), Milan GELNAR (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic)

Edition

International Symposium on Flatworm Biology, 2018

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10600 1.6 Biological sciences

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/18:00101136

Organization unit

Faculty of Science

Keywords in English

Monogenea; Eudiplozoon; nipponicum; excretory; secretory; genome; transcriptome
Změněno: 5/9/2018 15:43, Mgr. Jiří Vorel, Ph.D.

Abstract

V originále

Ectoparasitic flatworms from the class Monogenea represent serious fish pathogens and their presence in stocks can lead to significant losses in fish host populations. Despite this fact, the running research has been focused mainly on morphological and phylogenetical characteristics of these worms and the information related to the biochemical and molecular nature of the physiological processes is rather sporadic, as well as the knowledge of the molecules produced by monogeneans and their role in host-parasite interaction. From a large group of Monogenea only a few monogenean protein molecules have been deeply molecularly/biochemically characterized, e.g. peptidases (cathepsins B, D and L) and their inhibitors (cystatins and serpins), thioredoxin and annexin. Up to this date only two monogenean species were deeply sequenced on whole-genomic level. Genomes of Gyrodactylus salaris (Monopisthocotylea: Gyrodactylidae) and Protopolystoma xenopodis (Polyopisthocotylea: Polystomatidae) are currently available in public sequence databases. We would like to change this situation and therefore we adopted Eudiplozoon nipponicum from second large group of Monogenea Polyopisthocotylea (Diplozoidae) as an experimental organism for purpose recognize the important physiological pathways and crucial protein molecules involved in interactions with the fish host. E. nipponicum is a blood-feeding parasite inhabiting gills predominantly of common carp Cyprinus carpio, which is economically very important fish species in the Czech Republic. We started with analyses leaded to generation of E. nipponicum genome, transcriptome and secretome database. For the first E. nipponicum genome draft we used three sequencing techniques. 454/Roche sequencing (Junior platform), Illumina sequencing (MiSeq and HiSeq platforms) and Oxford Nanopore sequencing (MinION platform). 165,871,596 genomic raw reads were generated in total and after quality processing (trimming, removing contaminants, errors correction, etc.), 130,741,241 reads were used for the draft of genome assembly. Currently, the mitochondrial genome was already reconstructed and further bioinformatic evaluation of obtained assembled data is in progress. Generating of transcriptomic/secretomic dataset was based on usage of two sequencing methods (454/Roche and Illumina MiSeq platform) and mass spectrometry analysis (HPLC MS/MS, Orbitrap Elite Hybrid spectrometer). Total RNA, in the form of cDNA, was sequenced and raw reads were processed and assembled using specific bioinformatic tools. Based on the statistical evaluation and post-filtering steps, final transcriptome database contains 37,062 contaminant-free transcripts with corresponding translated NUMBER proteins. During annotation step, 19,539 (52.7 %) transcripts were homologous to some record in selected public sequence databases and 18,556 (50.1 %) of them were linked with sequences related to organisms only in phylum Platyhelminthes deposited in UniProtKB/TrEMBL database. Excretory-secretory products (ESP), protein compounds present in parasite secretome, were obtained from ~100 living adults and analysis of secretome peptide mixture revealed 1,033 proteins.

Links

GBP505/12/G112, research and development project
Name: ECIP - Evropské centrum ichtyoparazitologie
Investor: Czech Science Foundation
MUNI/A/0816/2017, interní kód MU
Name: Výzkum ekologicko-evolučních vztahů bezobratlých živočichů (Acronym: EKOLINKS)
Investor: Masaryk University, Category A
MUNI/A/1362/2016, interní kód MU
Name: Biologická diverzita – analýzy biologických systémů různých úrovní a na různých škálách prostředí
Investor: Masaryk University, Category A