Immunosensors and immunoassays for detection of clinically
important analytes

p 2018

Immunosensors and immunoassays for detection of clinically important analytes

FARKA, Zdeněk

Basic information

Original name

Immunosensors and immunoassays for detection of clinically important analytes

Authors

FARKA, Zdeněk

Edition

Invited lecture at Institute of Macromolecular Chemistry AS CR, 2018

Other information

Language

English

Type of outcome

Vyžádané přednášky

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Central European Institute of Technology

Keywords in English

Biosensor; Immunosensor; Immunoassay; Nanoparticle; Single-molecule detection

Změněno: 8/9/2018 10:35, doc. Mgr. Zdeněk Farka, Ph.D.

Abstract

V originále

Highly sensitive and specific bioanalysis is essential in all fields of human activities from food industry to clinical diagnostics. Among many analytical techniques, immunoassays and immunosensors are gaining attention due to the high specificity provided by antibodies and excellent sensitivity provided by various labels. We have employed immunosensors for the detection of Salmonella Typhimurium, which belongs to leading agents of gastrointestinal diseases. The point-of-care sensor was based on electrochemical impedance spectroscopy and screen-printed electrodes modified by specific antibody. We have further developed a signal enhancement by enzymatic precipitation for highly sensitive laboratory-based detection of Salmonella using surface plasmon resonance. Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals, which convert near-infrared light to light of shorter wavelengths. We have exploited UCNPs as a label in upconversion-linked immunoassay for the detection of pharmaceutical diclofenac, which is a common micropollutant in waters. The low background and high photostability make UCNPs a powerful tool for detection of single molecules. We have developed an optical approach for visualizing individual UCNPs and applied it for the sensitive detection of the cancer biomarker prostate specific antigen. The noise-surpassing digital readout provided single particle resolution with almost no instrumental background.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

FARKA, Zdeněk. Immunosensors and immunoassays for detection of clinically important analytes. In Invited lecture at Institute of Macromolecular Chemistry AS CR. 2018.

@misc{1433476,
   author = {Farka, Zdeněk},
   booktitle = {Invited lecture at Institute of Macromolecular Chemistry AS CR},
   keywords = {Biosensor; Immunosensor; Immunoassay; Nanoparticle; Single-molecule detection},
   language = {eng},
   title = {Immunosensors and immunoassays for detection of clinically important analytes},
   year = {2018}
}

TY  - SLIDE
ID  - 1433476
AU  - Farka, Zdeněk
PY  - 2018
TI  - Immunosensors and immunoassays for detection of clinically important analytes
KW  - Biosensor
KW  - Immunosensor
KW  - Immunoassay
KW  - Nanoparticle
KW  - Single-molecule detection
N2  - Highly sensitive and specific bioanalysis is essential in all fields of human activities from food industry to clinical diagnostics. Among many analytical techniques, immunoassays and immunosensors are gaining attention due to the high specificity provided by antibodies and excellent sensitivity provided by various labels. We have employed immunosensors for the detection of Salmonella Typhimurium, which belongs to leading agents of gastrointestinal diseases. The point-of-care sensor was based on electrochemical impedance spectroscopy and screen-printed electrodes modified by specific antibody. We have further developed a signal enhancement by enzymatic precipitation for highly sensitive laboratory-based detection of Salmonella using surface plasmon resonance. Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals, which convert near-infrared light to light of shorter wavelengths. We have exploited UCNPs as a label in upconversion-linked immunoassay for the detection of pharmaceutical diclofenac, which is a common micropollutant in waters. The low background and high photostability make UCNPs a powerful tool for detection of single molecules. We have developed an optical approach for visualizing individual UCNPs and applied it for the sensitive detection of the cancer biomarker prostate specific antigen. The noise-surpassing digital readout provided single particle resolution with almost no instrumental background.
ER  -

FARKA, Zdeněk. Immunosensors and immunoassays for detection of clinically important analytes. In \textit{Invited lecture at Institute of Macromolecular Chemistry AS CR}. 2018.

Detailed Information on Publication Record