Magnet Activated Cell Sorting Method in Sperm Selection and
Their Benefit in Human Assisted Reproduction

J 2018

Magnet Activated Cell Sorting Method in Sperm Selection and Their Benefit in Human Assisted Reproduction

VENTRUBA, Pavel, Michal JEŠETA, Jana ŽÁKOVÁ, B. KEMPISTY, Eva CHMELÍKOVÁ et. al.

Basic information

Original name

Magnet Activated Cell Sorting Method in Sperm Selection and Their Benefit in Human Assisted Reproduction

Authors

VENTRUBA, Pavel (203 Czech Republic, belonging to the institution), Michal JEŠETA (203 Czech Republic, guarantor, belonging to the institution), Jana ŽÁKOVÁ (203 Czech Republic, belonging to the institution), B. KEMPISTY (616 Poland), Eva CHMELÍKOVÁ (203 Czech Republic) and Igor CRHA (203 Czech Republic, belonging to the institution)

Edition

Biomedical journal of scientific & technical research, New York, Biomedical Research Network+ 2018, 2574-1241

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/18:00103646

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.26717/BJSTR.2018.06.001378

Keywords in English

Spermatozoa; MACS; DNA integrity;Swim-up;Apoptosis; Sperm preparation

Abstract

V originále

Center of Assisted Reproduction in University Hospital Brno is the oldest one in Czech Republic and is linked to academic institution, Masaryk University Brno. We have long time experiences with the therapy of human infertility. During last years we are focused on evaluation of novel techniques in assisted reproduction. Sperm selection for assisted reproductive techniques (ART) is very important step for successful in vitro fertilization [l-6].Classical ICSI (introcytoplasmatic sperm injection) method is not optimal for subjective sperm selection based on only morphology and motility of spermatozoa. For this reason there is currently a trend to combine multiple methods to segregate inappropriate sperm based on multiple parameters to increase the likelihood of successful fertilization. Is it usually classical separation method like swim-up or density gradient and a group of additional techniques like PICSI (ICSI with preselected sperm). One of these additional methods is MACS (magnet activated cell sorting). How many methods are best combined, and whether a new MACS selection method really brings a better prognosis in terms of conception success, or from the point of view of the subsequent development of the foetus, has already been studied several times.

Links

ROZV/24/LF/2016, interní kód MU

Name: LF - Příspěvek IP 2016

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

VENTRUBA, Pavel, Michal JEŠETA, Jana ŽÁKOVÁ, B. KEMPISTY, Eva CHMELÍKOVÁ and Igor CRHA. Magnet Activated Cell Sorting Method in Sperm Selection and Their Benefit in Human Assisted Reproduction. Biomedical journal of scientific & technical research. New York: Biomedical Research Network+, 2018, vol. 6, No 4, p. 1-2. ISSN 2574-1241. Available from: https://dx.doi.org/10.26717/BJSTR.2018.06.001378.

@article{1435147,
   author = {Ventruba, Pavel and Ješeta, Michal and Žáková, Jana and Kempisty, B. and Chmelíková, Eva and Crha, Igor},
   article_location = {New York},
   article_number = {4},
   doi = {http://dx.doi.org/10.26717/BJSTR.2018.06.001378},
   keywords = {Spermatozoa; MACS; DNA integrity;Swim-up;Apoptosis; Sperm preparation},
   language = {eng},
   issn = {2574-1241},
   journal = {Biomedical journal of scientific & technical research},
   title = {Magnet Activated Cell Sorting Method in Sperm Selection and Their Benefit in Human Assisted Reproduction},
   volume = {6},
   year = {2018}
}

TY  - JOUR
ID  - 1435147
AU  - Ventruba, Pavel - Ješeta, Michal - Žáková, Jana - Kempisty, B. - Chmelíková, Eva - Crha, Igor
PY  - 2018
TI  - Magnet Activated Cell Sorting Method in Sperm Selection and Their Benefit in Human Assisted Reproduction
JF  - Biomedical journal of scientific & technical research
VL  - 6
IS  - 4
SP  - 1-2
EP  - 1-2
PB  - Biomedical Research Network+
SN  - 25741241
KW  - Spermatozoa
KW  - MACS
KW  - DNA integrity;Swim-up;Apoptosis
KW  - Sperm preparation
N2  - Center of Assisted Reproduction in University Hospital Brno is the oldest one in Czech Republic and is linked to academic institution, Masaryk University Brno. We have long time experiences with the therapy of human infertility. During last years we are focused on evaluation of novel techniques in assisted reproduction. Sperm selection for assisted reproductive techniques (ART) is very important step for successful in vitro fertilization [l-6].Classical ICSI (introcytoplasmatic sperm injection) method is not optimal for subjective sperm selection based on only morphology and motility of spermatozoa. For this reason there is currently a trend to combine multiple methods to segregate inappropriate sperm based on multiple parameters to increase the likelihood of successful fertilization. Is it usually classical separation method like swim-up or density gradient and a group of additional techniques like PICSI (ICSI with preselected sperm). One of these additional methods is MACS (magnet activated cell sorting). How many methods are best combined, and whether a new MACS selection method really brings a better prognosis in terms of conception success, or from the point of view of the subsequent development of the foetus, has already been studied several times.
ER  -

VENTRUBA, Pavel, Michal JEŠETA, Jana ŽÁKOVÁ, B. KEMPISTY, Eva CHMELÍKOVÁ and Igor CRHA. Magnet Activated Cell Sorting Method in Sperm Selection and Their Benefit in Human Assisted Reproduction. \textit{Biomedical journal of scientific \&{} technical research}. New York: Biomedical Research Network+, 2018, vol.~6, No~4, p.~1-2. ISSN~2574-1241. Available from: https://dx.doi.org/10.26717/BJSTR.2018.06.001378.

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