Cyclopropylamine plasma polymer surfaces for label-free SPR and
QCM immunosensing of Salmonella

J 2018

Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella

MAKHNEVA, Ekaterina, Zdeněk FARKA, Petr SKLÁDAL and Lenka ZAJÍČKOVÁ

Basic information

Original name

Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella

Authors

MAKHNEVA, Ekaterina (643 Russian Federation, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Sensors and Actuators B: Chemical, Amsterdam, Elsevier B.V. 2018, 0925-4005

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.393

RIV identification code

RIV/00216224:14740/18:00101169

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.snb.2018.08.055

UT WoS

000444637600056

Keywords in English

Plasma polymerization; Amine films; Surface plasmon resonance; Quartz crystal microbalance; Immunosensor; Label-free detection

Abstract

V originále

We report on the first successful application of nitrogen-containing functional surfaces prepared by plasma polymerization in surface plasmon resonance (SPR) immunosensing. The plasma polymers (PPs) were deposited from cyclopropylamine (CPA) vapors onto gold surfaces of SPR and quartz crystal microbalance (QCM) sensors. It provided an excellent platform for a stable immobilization of antibodies using glutaraldehyde (GA) activation. The performance of the SPR immunosensor was proven by a model pair of monoclonal antibody (Ab) AL-01 and human serum albumin as well as by the selective detection of bacterial pathogen Salmonella Typhimurium using the immobilized anti-Salmonella Ab. A baseline drift of the measured SPR signal, caused by the long-term reactivity of CPA plasma polymers, was well stabilized by 18 h immersion in phosphate buffered saline prior to the GA activation. The immunosensors can be successfully regenerated several times using 10 mM NaOH. The limit of detection of 10^5 CFU/mL and a wide linear response were achieved for Salmonella. The assay parameters are comparable to the conventional label-free methods. However, the replacement of conventional immobilization matrix with the CPA PPs offers an advantage of excellent adhesion to gold QCM and SPR surfaces achieved by fast and eco-friendly procedure compatible with the vacuum deposition of gold.

Links

GA18-12774S, research and development project

Name: Plazmové polymery připravené na nanovlákenných membránách pro inženýrství cévní tkáně

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MAKHNEVA, Ekaterina, Zdeněk FARKA, Petr SKLÁDAL and Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella. Sensors and Actuators B: Chemical. Amsterdam: Elsevier B.V., 2018, vol. 276, DEC, p. 447-455. ISSN 0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2018.08.055.

@article{1436440,
   author = {Makhneva, Ekaterina and Farka, Zdeněk and Skládal, Petr and Zajíčková, Lenka},
   article_location = {Amsterdam},
   article_number = {DEC},
   doi = {http://dx.doi.org/10.1016/j.snb.2018.08.055},
   keywords = {Plasma polymerization; Amine films; Surface plasmon resonance; Quartz crystal microbalance; Immunosensor; Label-free detection},
   language = {eng},
   issn = {0925-4005},
   journal = {Sensors and Actuators B: Chemical},
   title = {Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella},
   url = {https://www.sciencedirect.com/science/article/pii/S0925400518314680},
   volume = {276},
   year = {2018}
}

TY  - JOUR
ID  - 1436440
AU  - Makhneva, Ekaterina - Farka, Zdeněk - Skládal, Petr - Zajíčková, Lenka
PY  - 2018
TI  - Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella
JF  - Sensors and Actuators B: Chemical
VL  - 276
IS  - DEC
SP  - 447-455
EP  - 447-455
PB  - Elsevier B.V.
SN  - 09254005
KW  - Plasma polymerization
KW  - Amine films
KW  - Surface plasmon resonance
KW  - Quartz crystal microbalance
KW  - Immunosensor
KW  - Label-free detection
UR  - https://www.sciencedirect.com/science/article/pii/S0925400518314680
N2  - We report on the first successful application of nitrogen-containing functional surfaces prepared by plasma polymerization in surface plasmon resonance (SPR) immunosensing. The plasma polymers (PPs) were deposited from cyclopropylamine (CPA) vapors onto gold surfaces of SPR and quartz crystal microbalance (QCM) sensors. It provided an excellent platform for a stable immobilization of antibodies using glutaraldehyde (GA) activation. The performance of the SPR immunosensor was proven by a model pair of monoclonal antibody (Ab) AL-01 and human serum albumin as well as by the selective detection of bacterial pathogen Salmonella Typhimurium using the immobilized anti-Salmonella Ab. A baseline drift of the measured SPR signal, caused by the long-term reactivity of CPA plasma polymers, was well stabilized by 18 h immersion in phosphate buffered saline prior to the GA activation. The immunosensors can be successfully regenerated several times using 10 mM NaOH. The limit of detection of 10^5 CFU/mL and a wide linear response were achieved for Salmonella. The assay parameters are comparable to the conventional label-free methods. However, the replacement of conventional immobilization matrix with the CPA PPs offers an advantage of excellent adhesion to gold QCM and SPR surfaces achieved by fast and eco-friendly procedure compatible with the vacuum deposition of gold.
ER  -

MAKHNEVA, Ekaterina, Zdeněk FARKA, Petr SKLÁDAL and Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella. \textit{Sensors and Actuators B: Chemical}. Amsterdam: Elsevier B.V., 2018, vol.~276, DEC, p.~447-455. ISSN~0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2018.08.055.

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