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@article{1439376,
author = {Jarošová, Petra and Paroulek, Petr and Rájecký, Michal and Rájecká, Veronika and Táborská, Eva and Eritja, R. and Avino, A. and Mazzini, S. and Gargallo, R. and Táborský, Petr},
article_location = {CAMBRIDGE},
article_number = {33},
doi = {http://dx.doi.org/10.1039/c8cp02681e},
keywords = {HUMAN TELOMERIC DNA; ANTIPROLIFERATIVE ACTIVITY; PROMOTER REGION; SMALL MOLECULES; DRUG DESIGN; K+ SOLUTION; IN-VIVO; BINDING; BENZOPHENANTHRIDINE; OLIGONUCLEOTIDES},
language = {eng},
issn = {1463-9076},
journal = {Physical Chemistry Chemical Physics},
title = {Naturally occurring quaternary benzo[c]phenanthridine alkaloids selectively stabilize G-quadruplexes},
url = {http://pubs.rsc.org/en/Content/ArticleLanding/2018/CP/C8CP02681E#!divAbstract},
volume = {20},
year = {2018}
}
TY - JOUR
ID - 1439376
AU - Jarošová, Petra - Paroulek, Petr - Rájecký, Michal - Rájecká, Veronika - Táborská, Eva - Eritja, R. - Avino, A. - Mazzini, S. - Gargallo, R. - Táborský, Petr
PY - 2018
TI - Naturally occurring quaternary benzo[c]phenanthridine alkaloids selectively stabilize G-quadruplexes
JF - Physical Chemistry Chemical Physics
VL - 20
IS - 33
SP - 21772-21782
EP - 21772-21782
PB - Royal Society of Chemistry
SN - 14639076
KW - HUMAN TELOMERIC DNA
KW - ANTIPROLIFERATIVE ACTIVITY
KW - PROMOTER REGION
KW - SMALL MOLECULES
KW - DRUG DESIGN
KW - K+ SOLUTION
KW - IN-VIVO
KW - BINDING
KW - BENZOPHENANTHRIDINE
KW - OLIGONUCLEOTIDES
UR - http://pubs.rsc.org/en/Content/ArticleLanding/2018/CP/C8CP02681E#!divAbstract
N2 - In this work, the interaction of six natural benzo[c]phenanthridine alkaloids (macarpine, sanguilutine, sanguirubine, chelerythrine, sanguinarine and chelirubine) with parallel and antiparallel G-quadruplex DNA structures was studied. HT22 corresponding to the end of human telomeres and the modified promoter oncogene c-kit21 and Pu22 sequences have been used. Spectroscopically-monitored melting experiments and fluorescence titrations, competitive dialysis and nuclear magnetic resonance spectroscopy were used for this purpose. The results showed that these alkaloids stabilized G-quadruplex structures in terms of increments of T-m values (from 15 to 25 degrees C) with high selectivity over duplexes and unfolded DNA. The mode of binding was mainly by stacking on the terminal G-tetrads with stoichiometries of 1:2 (DNA:ligand). The presence of non-specific electrostatic interactions was also observed. Overall, the results pointed to a strong stabilization of G-quadruplex structures by these alkaloids.
ER -
JAROŠOVÁ, Petra, Petr PAROULEK, Michal RÁJECKÝ, Veronika RÁJECKÁ, Eva TÁBORSKÁ, R. ERITJA, A. AVINO, S. MAZZINI, R. GARGALLO and Petr TÁBORSKÝ. Naturally occurring quaternary benzo[c]phenanthridine alkaloids selectively stabilize G-quadruplexes. \textit{Physical Chemistry Chemical Physics}. CAMBRIDGE: Royal Society of Chemistry, 2018, vol.~20, No~33, p.~21772-21782. ISSN~1463-9076. Available from: https://dx.doi.org/10.1039/c8cp02681e.
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