Polymorphisms in genes linked with folate metabolic pathway in Czech children with dental caries

BOŘILOVÁ LINHARTOVÁ, Petra, Jana MRÁZKOVÁ, David NOVÁK, Dominika HULOVÁ, Lubomír KUKLA, Martina KUKLETOVÁ and Lydie IZAKOVIČOVÁ HOLLÁ. Polymorphisms in genes linked with folate metabolic pathway in Czech children with dental caries. In HGSA 2018, Sydney, Australia. 2018.

Basic information

• Original name: Polymorphisms in genes linked with folate metabolic pathway in Czech children with dental caries
• Authors: BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, belonging to the institution), Jana MRÁZKOVÁ (203 Czech Republic), David NOVÁK (203 Czech Republic), Dominika HULOVÁ (703 Slovakia), Lubomír KUKLA (203 Czech Republic), Martina KUKLETOVÁ (203 Czech Republic) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic).
• Edition: HGSA 2018, Sydney, Australia, 2018.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30101 Human genetics
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/18:00101192
• Organization unit: Faculty of Medicine
• Keywords in English: folate; gene polymorphism; children; oral status; case-control study

Abstract

Background: Dental caries is a multifactorial, infectious disease where genetic predisposition can play an important role. Its susceptibility may be modified by functional polymorphisms in genes linked with the folate metabolic pathway, such as methionine synthase (MTR), methionine synthase reductase (MTRR), and methylenetetrahydrofolate reductase (MTHFR). The aim of this study was to analyze four single nucleotide polymorphisms (SNPs) in the genes for these enzymes in the group of caries free children vs. subjects affected by dental caries in the Czech population. Methods: This case-control study included 1017 subjects: 145 caries free children (with decayed/missing/filled teeth DMFT=0) and 466 subjects with dental caries (DMFT 1 or higher) aged 13-15 years from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) and 282 children aged 2-6 years with early childhood caries (ECC, dmft 1 or higher) together with 124 caries free children (dmft=0). MTHFR C677T (rs1801133), MTHFR A1298C (rs1801131), MTR A2756G (rs1805087) and MTRR A66G (rs1801394) SNPs were determined by qPCR. Results: Although no significant differences in the allele or genotype frequencies of all four SNPs between the caries free children and those affected by ECC were observed (P higher than 0.05), MTHFR 1298 AA vs. AC+CC and MTHFR 677 TT vs. CT+CC genotypes were found as protective factor for dental caries (P lower than 0.05 and P lower than 0.01, respectively) in children with permanent dentition. There were no significant differences in the allele or genotype frequencies in both MTR and MTRR SNPs between these patients and healthy controls (P higher than 0.05). Conclusions: MTHFR polymorphisms may be associated with caries protection in permanent but not primary dentition.

Links

• GB14-37368G, research and development project: Name: Centrum orofaciálního vývoje a regenerace

Investor: Czech Science Foundation

• MUNI/A/1008/2017, interní kód MU: Name: Etiopatogenetické aspekty, diagnostika a léčba vybraných onemocnění dutiny ústní

Investor: Masaryk University, Category A

• NV17-30439A, research and development project: Name: Moderní biotechnologické a behaviorální přístupy ve výzkumu zubního kazu a strategie jeho prevence
• ROZV/24/LF/2018, interní kód MU: Name: LF - Příspěvek na IP 2108

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects