Genetic analysis of a relation between SMC5/6 and SAGA
chromatin modifying complex

a 2018

Genetic analysis of a relation between SMC5/6 and SAGA chromatin modifying complex

JURČIŠINOVÁ, Lenka, Jin WANG, Lucia MOLNÁROVÁ, Silvia BÁGEĽOVÁ POLÁKOVÁ, Juraj GREGÁŇ et. al.

Basic information

Original name

Genetic analysis of a relation between SMC5/6 and SAGA chromatin modifying complex

Authors

JURČIŠINOVÁ, Lenka, Jin WANG, Lucia MOLNÁROVÁ, Silvia BÁGEĽOVÁ POLÁKOVÁ, Juraj GREGÁŇ, Christian HAERING, Jiří FAJKUS and Jan PALEČEK

Edition

Genetická konferencia GSGM, 2018

Other information

Type of outcome

Konferenční abstrakt

Confidentiality degree

není předmětem státního či obchodního tajemství

ISBN

978-80-223-4545-3

Abstract

V originále

The SMC complexes (cohesin, condensin and SMC5/6 complex) are highly conserved molecular machineries and the key components of higher order chromatin structures. The cohesin complex mediates sister chromatid cohesion from S-phase until anaphase, whilst condensin promotes chromatin condensation during mitosis. The SMC5/6 complex is essential for proliferation in yeast and is involved in the non-essential homologous recombination-based DNA repair processes, including repair of DNA double strand breaks, restart of stalled replication forks etc. However the precise mechanisms of its involvement in these biological processes remain unclear. To uncover its essential function, we decided to do yeast S.pombe synthetic lethal genetic screen with hypomorphic nse3/R254E DNA-binding mutation, which reduces association of the SMC5/6 complex with chromatin. Detailed genetic analysis revealed that HAT module of the SAGA H3 histone acetyltransferase complex negatively interacts with nse3/R254E and smc6 hypomorphic mutations. Using Y2H analysis, we found physical interactions between HAT module and SMC5/6 subunits. Genetic data suggest that the essential function of the SMC5/6 complex is likely connected with HAT module of the SAGA chromatin modifying complex.

Links

GA18-02067S, research and development project

Name: Úloha Nse1/Nse3/Nse4, E3-ubikvitin ligásy, ve stabilitě genomu

Investor: Czech Science Foundation

Citovat

JURČIŠINOVÁ, Lenka, Jin WANG, Lucia MOLNÁROVÁ, Silvia BÁGEĽOVÁ POLÁKOVÁ, Juraj GREGÁŇ, Christian HAERING, Jiří FAJKUS and Jan PALEČEK. Genetic analysis of a relation between SMC5/6 and SAGA chromatin modifying complex. In Genetická konferencia GSGM. 2018. ISBN 978-80-223-4545-3.

@proceedings{1443278,
   author = {Jurčišinová, Lenka and Wang, Jin and Molnárová, Lucia and Bágeľová Poláková, Silvia and Gregáň, Juraj and Haering, Christian and Fajkus, Jiří and Paleček, Jan},
   booktitle = {Genetická konferencia GSGM},
   isbn = {978-80-223-4545-3},
   title = {Genetic analysis of a relation between SMC5/6 and SAGA chromatin modifying complex},
   year = {2018}
}

TY  - CONF
ID  - 1443278
AU  - Jurčišinová, Lenka - Wang, Jin - Molnárová, Lucia - Bágeľová Poláková, Silvia - Gregáň, Juraj - Haering, Christian - Fajkus, Jiří - Paleček, Jan
PY  - 2018
TI  - Genetic analysis of a relation between SMC5/6 and SAGA chromatin modifying complex
SN  - 9788022345453
N2  - The SMC complexes (cohesin, condensin and SMC5/6 complex) are highly conserved molecular machineries and the key components of higher order chromatin structures. The cohesin complex mediates sister chromatid cohesion from S-phase until anaphase, whilst condensin promotes chromatin condensation during mitosis. The SMC5/6 complex is essential for proliferation in yeast and is involved in the non-essential homologous recombination-based DNA repair processes, including repair of DNA double strand breaks, restart of stalled replication forks etc. However the precise mechanisms of its involvement in these biological processes remain unclear. To uncover its essential function, we decided to do yeast S.pombe synthetic lethal genetic screen with hypomorphic nse3/R254E DNA-binding mutation, which reduces association of the SMC5/6 complex with chromatin. Detailed genetic analysis revealed that HAT module of the SAGA H3 histone acetyltransferase complex negatively interacts with nse3/R254E and smc6 hypomorphic mutations. Using Y2H analysis, we found physical interactions between HAT module and SMC5/6 subunits. Genetic data suggest that the essential function of the SMC5/6 complex is likely connected with HAT module of the SAGA chromatin modifying complex.
ER  -

JURČIŠINOVÁ, Lenka, Jin WANG, Lucia MOLNÁROVÁ, Silvia BÁGEĽOVÁ POLÁKOVÁ, Juraj GREGÁŇ, Christian HAERING, Jiří FAJKUS and Jan PALEČEK. Genetic analysis of a relation between SMC5/6 and SAGA chromatin modifying complex. In \textit{Genetická konferencia GSGM}. 2018. ISBN~978-80-223-4545-3.

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