J 2018

Cytokinin signalling regulates organ identity via AHK4 receptor in Arabidopsis.

PERNISOVÁ, Markéta, Martina GROCHOVÁ, Tomáš KONEČNÝ, Lenka PLAČKOVÁ, Danka HARUŠTIAKOVÁ et. al.

Basic information

Original name

Cytokinin signalling regulates organ identity via AHK4 receptor in Arabidopsis.

Authors

PERNISOVÁ, Markéta (203 Czech Republic, belonging to the institution), Martina GROCHOVÁ (203 Czech Republic), Tomáš KONEČNÝ (203 Czech Republic, belonging to the institution), Lenka PLAČKOVÁ (203 Czech Republic), Danka HARUŠTIAKOVÁ (703 Slovakia, belonging to the institution), Tatsuo KAKIMOTO (392 Japan), Marcus HEISLER (276 Germany), Ondřej NOVÁK (203 Czech Republic) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution)

Edition

Development, 2018, 0950-1991

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10605 Developmental biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.763

RIV identification code

RIV/00216224:14740/18:00101227

Organization unit

Central European Institute of Technology

DOI

UT WoS

000440421300010

Keywords in English

Arabidopsis; Cytokinin metabolism; Cytokinin signalling; De novo organogenesis; Root; Shoot

Tags

Tags

International impact, Reviewed
Změněno: 14/11/2024 14:15, Mgr. Eva Dubská

Abstract

V originále

Mutual interactions of the phytohormones cytokinins and auxin determine root or shoot identity during postembryonic de novo organogenesis in plants. However, our understanding to the role of hormonal metabolism and perception during early stages of cell fate reprograming is still elusive. In the hypocotyl explant assay, auxin activated root formation while cytokinins mediated early loss of the root identity, primordia disorganization and initiation of shoot development. Exogenous but also endogenous cytokinins influenced the initiation of newly formed organs as well as the pace of organ developmental sequence. The process of de novo shoot apical meristem establishment was accompanied by accumulation of endogenous cytokinins, differential regulation of genes for individual cytokinin receptors, strong activation of AHK4-mediated signalling and induction of shoot-specific homeodomain regulator WUSCHEL. The latter associated with upregulation of isopentenyladenine-type cytokinins, revealing higher shoot-forming potential when compared with trans-zeatin. Moreover, AHK4-controlled cytokinin signalling negatively regulated root stem cell organizer WUSCHEL RELATED HOMEOBOX 5 in the root quiescent centre. We propose an important role of endogenous cytokinin biosynthesis and AHK4-mediated cytokinin signalling in the control of de novo induced organ identity.

Links

GA13-25280S, research and development project
Name: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development
Investor: Czech Science Foundation
GP14-30004P, research and development project
Name: Cytokininy řízené molekulární mechanizmy regulující identitu orgánů u Arabidopsis.
Investor: Czech Science Foundation
LH14104, research and development project
Name: Zavedení zobrazovací metody in vivo pro analýzu molekulárních mechanizmů regulace buněčného dělení a diferenciace kořene Arabidopsis thaliana
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
90062, large research infrastructures
Name: Czech-BioImaging