KOSTOLANSKÁ, Katarína, Lucie NOVOTNÁ, Eva TÁBORSKÁ and Ondřej PEŠ. Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte. Chemical Papers. Cham: SPRINGER INTERNATIONAL PUBLISHING AG, 2019, vol. 73, No 1, p. 151-158. ISSN 0366-6352. Available from: https://dx.doi.org/10.1007/s11696-018-0560-1.
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Basic information
Original name Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte
Authors KOSTOLANSKÁ, Katarína (703 Slovakia, belonging to the institution), Lucie NOVOTNÁ (203 Czech Republic, belonging to the institution), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution) and Ondřej PEŠ (203 Czech Republic, guarantor, belonging to the institution).
Edition Chemical Papers, Cham, SPRINGER INTERNATIONAL PUBLISHING AG, 2019, 0366-6352.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10609 Biochemical research methods
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.963 in 2017
RIV identification code RIV/00216224:14110/19:00108893
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s11696-018-0560-1
UT WoS 000458121900014
Keywords in English Endogenous cortisol; Long-term hair concentration; Surrogate analyte LC–MS; Online SPE
Tags 14110512, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 11/5/2020 08:14.
Abstract
Online SPE LC–MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.
Links
MUNI/A/0910/2017, interní kód MUName: Studium molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: stumopo)
Investor: Masaryk University, Category A
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