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@article{1450136,
author = {Mazalova, L. and Sladek, Z. and Raudenská, Martina and Balvan, Jan and Gumulec, Jaromír and Masařík, Michal},
article_location = {BRATISLAVA},
article_number = {8},
doi = {http://dx.doi.org/10.4149/BLL_2018_095},
keywords = {prostate; cancer; supernatant; macrophages; cytokines},
language = {eng},
issn = {0006-9248},
journal = {Bratislava Medical Journal - Bratislavské lekárske listy},
title = {Effect of prostate cancer cell line supernatant on functional polarization in macrophages},
volume = {119},
year = {2018}
}
TY - JOUR
ID - 1450136
AU - Mazalova, L. - Sladek, Z. - Raudenská, Martina - Balvan, Jan - Gumulec, Jaromír - Masařík, Michal
PY - 2018
TI - Effect of prostate cancer cell line supernatant on functional polarization in macrophages
JF - Bratislava Medical Journal - Bratislavské lekárske listy
VL - 119
IS - 8
SP - 516-521
EP - 516-521
PB - Univerzita Komenského
SN - 00069248
KW - prostate
KW - cancer
KW - supernatant
KW - macrophages
KW - cytokines
N2 - OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages. BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-inflammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-alpha). Macrophages exhibiting a phenotype M2 secrete anti-inflammatory cytokines, e.g. interleukin 10 (IL-10), transforming growth factor beta (TGF-beta). METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours. RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-alpha was measured by Quantitative real-time PCR. Expression of pro-inflammatory cytokines was lower in macrophages with supernatant compared to positive control. CONCLUSION: Expression of pro-inflammatory cytokines was lower in macrophages with supernatant (M phi+sup) compared to positive control (M phi+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-alpha was not confirmed (Tab. 1, Fig. 5, Ref. 15). Text in PDF www.elis.sk.
ER -
MAZALOVA, L., Z. SLADEK, Martina RAUDENSKÁ, Jan BALVAN, Jaromír GUMULEC and Michal MASAŘÍK. Effect of prostate cancer cell line supernatant on functional polarization in macrophages. \textit{Bratislava Medical Journal - Bratislavské lekárske listy}. BRATISLAVA: Univerzita Komenského, 2018, vol.~119, No~8, p.~516-521. ISSN~0006-9248. Available from: https://dx.doi.org/10.4149/BLL\_{}2018\_{}095.
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