J 2018

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

MAZALOVA, L., Z. SLADEK, Martina RAUDENSKÁ, Jan BALVAN, Jaromír GUMULEC et. al.

Basic information

Original name

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Authors

MAZALOVA, L. (203 Czech Republic), Z. SLADEK (203 Czech Republic, guarantor), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Jan BALVAN (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution) and Michal MASAŘÍK (203 Czech Republic, belonging to the institution)

Edition

Bratislava Medical Journal - Bratislavské lekárske listy, BRATISLAVA, Univerzita Komenského, 2018, 0006-9248

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.859

RIV identification code

RIV/00216224:14110/18:00104058

Organization unit

Faculty of Medicine

DOI

UT WoS

000444369100011

Keywords in English

prostate; cancer; supernatant; macrophages; cytokines

Tags

Tags

International impact, Reviewed
Změněno: 2/5/2019 14:10, Soňa Böhmová

Abstract

V originále

OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages. BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-inflammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-alpha). Macrophages exhibiting a phenotype M2 secrete anti-inflammatory cytokines, e.g. interleukin 10 (IL-10), transforming growth factor beta (TGF-beta). METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours. RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-alpha was measured by Quantitative real-time PCR. Expression of pro-inflammatory cytokines was lower in macrophages with supernatant compared to positive control. CONCLUSION: Expression of pro-inflammatory cytokines was lower in macrophages with supernatant (M phi+sup) compared to positive control (M phi+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-alpha was not confirmed (Tab. 1, Fig. 5, Ref. 15). Text in PDF www.elis.sk.