Calcium signaling mediates five types of cell morphological
changes to form neural rosettes

J 2018

Calcium signaling mediates five types of cell morphological changes to form neural rosettes

HŘÍBKOVÁ, Hana, Marta GRABIEC, Dobromila KLEMOVÁ, Iva SLANINOVÁ, Yuh-Man WADELEY et. al.

Basic information

Original name

Calcium signaling mediates five types of cell morphological changes to form neural rosettes

Authors

HŘÍBKOVÁ, Hana (203 Czech Republic, belonging to the institution), Marta GRABIEC (616 Poland, belonging to the institution), Dobromila KLEMOVÁ (203 Czech Republic, belonging to the institution), Iva SLANINOVÁ (203 Czech Republic, belonging to the institution) and Yuh-Man WADELEY (826 United Kingdom of Great Britain and Northern Ireland, guarantor, belonging to the institution)

Edition

Journal of Cell Science, Cambridge, Company of Biologists Ltd. 2018, 0021-9533

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.517

RIV identification code

RIV/00216224:14110/18:00104080

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1242/jcs.206896

UT WoS

000425917200007

Keywords in English

Embryonic stem cells; Neural stem cell niche; Neural rosette formation; Calcium; Neurogenesis; Adherens junctions; Cytoskeleton proteins

Abstract

V originále

Neural rosette formation is a critical morphogenetic process during neural development, whereby neural stem cells are enclosed in rosette niches to equipoise proliferation and differentiation. How neural rosettes form and provide a regulatory micro-environment remains to be elucidated. We employed the human embryonic stem cell-based neural rosette system to investigate the structural development and function of neural rosettes. Our study shows that neural rosette formation consists of five types of morphological change: intercalation, constriction, polarization, elongation and lumen formation. Ca2+ signaling plays a pivotal role in the five steps by regulating the actions of the cytoskeletal complexes, actin, myosin II and tubulin during intercalation, constriction and elongation. These, in turn, control the polarizing elements, ZO-1, PARD3 and beta-catenin during polarization and lumen production for neural rosette formation. We further demonstrate that the dismantlement of neural rosettes, mediated by the destruction of cytoskeletal elements, promotes neurogenesis and astrogenesis prematurely, indicating that an intact rosette structure is essential for orderly neural development.

Links

EE2.3.20.0011, research and development project

Name: Centrum výzkumu pluripotentních buněk a nestability genomu

MUNI/A/0810/2016, interní kód MU

Name: Molekulární a buněčná biologie

Investor: Masaryk University, Category A

NV15-31063A, research and development project

Name: Buněčné markery vedoucí ke specifické léčbě "na míru" schizofrenním pacientům

Citovat

HŘÍBKOVÁ, Hana, Marta GRABIEC, Dobromila KLEMOVÁ, Iva SLANINOVÁ and Yuh-Man WADELEY. Calcium signaling mediates five types of cell morphological changes to form neural rosettes. Journal of Cell Science. Cambridge: Company of Biologists Ltd., 2018, vol. 131, No 3, p. 1-12. ISSN 0021-9533. Available from: https://dx.doi.org/10.1242/jcs.206896.

@article{1451297,
   author = {Hříbková, Hana and Grabiec, Marta and Klemová, Dobromila and Slaninová, Iva and Wadeley, YuhandMan},
   article_location = {Cambridge},
   article_number = {3},
   doi = {http://dx.doi.org/10.1242/jcs.206896},
   keywords = {Embryonic stem cells; Neural stem cell niche; Neural rosette formation; Calcium; Neurogenesis; Adherens junctions; Cytoskeleton proteins},
   language = {eng},
   issn = {0021-9533},
   journal = {Journal of Cell Science},
   title = {Calcium signaling mediates five types of cell morphological changes to form neural rosettes},
   url = {http://dx.doi.org/10.1242/jcs.206896},
   volume = {131},
   year = {2018}
}

TY  - JOUR
ID  - 1451297
AU  - Hříbková, Hana - Grabiec, Marta - Klemová, Dobromila - Slaninová, Iva - Wadeley, Yuh-Man
PY  - 2018
TI  - Calcium signaling mediates five types of cell morphological changes to form neural rosettes
JF  - Journal of Cell Science
VL  - 131
IS  - 3
SP  - 1-12
EP  - 1-12
PB  - Company of Biologists Ltd.
SN  - 00219533
KW  - Embryonic stem cells
KW  - Neural stem cell niche
KW  - Neural rosette formation
KW  - Calcium
KW  - Neurogenesis
KW  - Adherens junctions
KW  - Cytoskeleton proteins
UR  - http://dx.doi.org/10.1242/jcs.206896
N2  - Neural rosette formation is a critical morphogenetic process during neural development, whereby neural stem cells are enclosed in rosette niches to equipoise proliferation and differentiation. How neural rosettes form and provide a regulatory micro-environment remains to be elucidated. We employed the human embryonic stem cell-based neural rosette system to investigate the structural development and function of neural rosettes. Our study shows that neural rosette formation consists of five types of morphological change: intercalation, constriction, polarization, elongation and lumen formation. Ca2+ signaling plays a pivotal role in the five steps by regulating the actions of the cytoskeletal complexes, actin, myosin II and tubulin during intercalation, constriction and elongation. These, in turn, control the polarizing elements, ZO-1, PARD3 and beta-catenin during polarization and lumen production for neural rosette formation. We further demonstrate that the dismantlement of neural rosettes, mediated by the destruction of cytoskeletal elements, promotes neurogenesis and astrogenesis prematurely, indicating that an intact rosette structure is essential for orderly neural development.
ER  -

HŘÍBKOVÁ, Hana, Marta GRABIEC, Dobromila KLEMOVÁ, Iva SLANINOVÁ and Yuh-Man WADELEY. Calcium signaling mediates five types of cell morphological changes to form neural rosettes. \textit{Journal of Cell Science}. Cambridge: Company of Biologists Ltd., 2018, vol.~131, No~3, p.~1-12. ISSN~0021-9533. Available from: https://dx.doi.org/10.1242/jcs.206896.

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