KOVACIK, Jozef, Marek BUJDOS and Petr BABULA. Impact of humic acid on the accumulation of metals by microalgae. Environmental Science and Pollution Research. Heidelberg: Springer Heidelberg, 2018, vol. 25, No 11, p. 10792-10798. ISSN 0944-1344. Available from: https://dx.doi.org/10.1007/s11356-018-1362-2.
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Basic information
Original name Impact of humic acid on the accumulation of metals by microalgae
Authors KOVACIK, Jozef (703 Slovakia, guarantor), Marek BUJDOS (703 Slovakia) and Petr BABULA (203 Czech Republic, belonging to the institution).
Edition Environmental Science and Pollution Research, Heidelberg, Springer Heidelberg, 2018, 0944-1344.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30105 Physiology
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.914
RIV identification code RIV/00216224:14110/18:00104103
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s11356-018-1362-2
UT WoS 000429810200056
Keywords in English Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress
Tags 14110515, rivok
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 10/2/2019 14:38.
Abstract
Indirect impact of humic acid (HA) on metal accumulation and toxicity (Cd, Ni, Pb, and Hg; 100 mu M; 24 h of exposure) in Scenedesmus quadricauda was studied. Algae were pre-cultured on solid (10 and 100 mg HA/L) or in liquid media (1, 5, and 10 mg HA/L) over 30 days and then exposed to metals mentioned above. Accumulation of applied metals irrespective of pre-culture increased in the order Ni < Cd < Pb < Hg. Algae pre-cultured on solid HA-enriched media accumulated more Cd (+ 46% at 10 mg HA/L), Ni (+ 50 and + 81% at 10 and 100 mg HA/L, respectively), and Pb (+ 15% at 100 mg HA/L) but the impact on Hg amount was not detected. Potassium and calcium decreased in response to all metals (K strongly under Hg excess) and HA had negligible impact. Interestingly, fluorescence microscopy detection of reactive oxygen species/nitric oxide (ROS/NO) balance showed that HA pre-culture suppressed ROS signal and stimulated NO signal in response to Cd (indicating positive impact of HA) while ROS signal in Ni and Pb treatments rather increased but NO signal decreased as expected from elevated Ni and Pb accumulation. Hg had clearly the most toxic impact on the ROS/NO balance. Algae pre-cultured in liquid HA-enriched media showed significantly increased Ni accumulation only (+ 14% at a dose 10 mg HA/L). Present study for the first time showed that humic acid may indirectly affect accumulation of metals and that solid HA-enriched medium used for pre-culture is more suitable to increase accumulation of metals by algae.
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