J 2018

Impact of humic acid on the accumulation of metals by microalgae

KOVACIK, Jozef, Marek BUJDOS and Petr BABULA

Basic information

Original name

Impact of humic acid on the accumulation of metals by microalgae

Authors

KOVACIK, Jozef (703 Slovakia, guarantor), Marek BUJDOS (703 Slovakia) and Petr BABULA (203 Czech Republic, belonging to the institution)

Edition

Environmental Science and Pollution Research, Heidelberg, Springer Heidelberg, 2018, 0944-1344

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.914

RIV identification code

RIV/00216224:14110/18:00104103

Organization unit

Faculty of Medicine

DOI

UT WoS

000429810200056

Keywords in English

Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress

Tags

Tags

International impact, Reviewed
Změněno: 10/2/2019 14:38, Soňa Böhmová

Abstract

V originále

Indirect impact of humic acid (HA) on metal accumulation and toxicity (Cd, Ni, Pb, and Hg; 100 mu M; 24 h of exposure) in Scenedesmus quadricauda was studied. Algae were pre-cultured on solid (10 and 100 mg HA/L) or in liquid media (1, 5, and 10 mg HA/L) over 30 days and then exposed to metals mentioned above. Accumulation of applied metals irrespective of pre-culture increased in the order Ni < Cd < Pb < Hg. Algae pre-cultured on solid HA-enriched media accumulated more Cd (+ 46% at 10 mg HA/L), Ni (+ 50 and + 81% at 10 and 100 mg HA/L, respectively), and Pb (+ 15% at 100 mg HA/L) but the impact on Hg amount was not detected. Potassium and calcium decreased in response to all metals (K strongly under Hg excess) and HA had negligible impact. Interestingly, fluorescence microscopy detection of reactive oxygen species/nitric oxide (ROS/NO) balance showed that HA pre-culture suppressed ROS signal and stimulated NO signal in response to Cd (indicating positive impact of HA) while ROS signal in Ni and Pb treatments rather increased but NO signal decreased as expected from elevated Ni and Pb accumulation. Hg had clearly the most toxic impact on the ROS/NO balance. Algae pre-cultured in liquid HA-enriched media showed significantly increased Ni accumulation only (+ 14% at a dose 10 mg HA/L). Present study for the first time showed that humic acid may indirectly affect accumulation of metals and that solid HA-enriched medium used for pre-culture is more suitable to increase accumulation of metals by algae.