Gene target selection for loop-mediated isothermal
amplification for rapid discrimination of Treponema pallidum
subspecies

J 2018

Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies

KNAUF, Sascha, Simone LUERT, David ŠMAJS, Michal STROUHAL, Idrissa S. CHUMA et. al.

Basic information

Original name

Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies

Authors

KNAUF, Sascha (276 Germany, guarantor), Simone LUERT (276 Germany), David ŠMAJS (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Idrissa S. CHUMA (276 Germany), Sieghard FRISCHMANN (276 Germany) and Mohammed BAKHEIT (276 Germany)

Edition

PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2018, 1935-2735

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30309 Tropical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.487

RIV identification code

RIV/00216224:14110/18:00104105

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pntd.0006396

UT WoS

000433487700036

Keywords in English

gene target

Abstract

V originále

We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings.

Citovat

KNAUF, Sascha, Simone LUERT, David ŠMAJS, Michal STROUHAL, Idrissa S. CHUMA, Sieghard FRISCHMANN and Mohammed BAKHEIT. Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. PLoS neglected tropical diseases. San Francisco: Public Library of Science, 2018, vol. 12, No 4, p. 1-14. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0006396.

@article{1452178,
   author = {Knauf, Sascha and Luert, Simone and Šmajs, David and Strouhal, Michal and Chuma, Idrissa S. and Frischmann, Sieghard and Bakheit, Mohammed},
   article_location = {San Francisco},
   article_number = {4},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0006396},
   keywords = {gene target},
   language = {eng},
   issn = {1935-2735},
   journal = {PLoS neglected tropical diseases},
   title = {Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies},
   volume = {12},
   year = {2018}
}

TY  - JOUR
ID  - 1452178
AU  - Knauf, Sascha - Luert, Simone - Šmajs, David - Strouhal, Michal - Chuma, Idrissa S. - Frischmann, Sieghard - Bakheit, Mohammed
PY  - 2018
TI  - Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies
JF  - PLoS neglected tropical diseases
VL  - 12
IS  - 4
SP  - 1-14
EP  - 1-14
PB  - Public Library of Science
SN  - 19352735
KW  - gene target
N2  - We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings.
ER  -

KNAUF, Sascha, Simone LUERT, David ŠMAJS, Michal STROUHAL, Idrissa S. CHUMA, Sieghard FRISCHMANN and Mohammed BAKHEIT. Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. \textit{PLoS neglected tropical diseases}. San Francisco: Public Library of Science, 2018, vol.~12, No~4, p.~1-14. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0006396.

Detailed Information on Publication Record