Endogenous H2S producing enzymes are involved in apoptosis
induction in clear cell renal cell carcinoma

J 2018

Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma

BREZA, Jan, Andrea SOLTYSOVA, Sona HUDECOVA, Adela PENESOVA, Ivan SZADVÁRI et. al.

Basic information

Original name

Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma

Authors

BREZA, Jan (703 Slovakia), Andrea SOLTYSOVA (703 Slovakia), Sona HUDECOVA (703 Slovakia), Adela PENESOVA (703 Slovakia), Ivan SZADVÁRI (703 Slovakia, belonging to the institution), Petr BABULA (203 Czech Republic, belonging to the institution), Barbora CHOVANCOVA (703 Slovakia), Lubomira LENCESOVA (703 Slovakia), Ondrej POS (703 Slovakia), Jan BREZA (703 Slovakia), Karol ONDRIAS (703 Slovakia) and Oľga KRIŽANOVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

BMC Cancer, London, BioMed Central, 2018, 1471-2407

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.933

RIV identification code

RIV/00216224:14110/18:00104139

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1186/s12885-018-4508-1

UT WoS

000433334000005

Keywords in English

Cystathionine-beta-synthase; Cystathionine gamma-lyase; 3-Mercaptopyruvate sulfurtransferase; Apoptosis; Clear cell renal cell carcinoma

Abstract

V originále

Background: Knowledge about the expression and thus a role of enzymes that produce endogenous H2S-cystathionine-beta-synthase, cystathionine gamma-lyase and mercaptopyruvate sulfurtransferase - in renal tumors is still controversial. In this study we aimed to determine the expression of these enzymes relatively to the expression in unaffected part of kidney from the same patient and to found relation of these changes to apoptosis. To evaluate patient's samples, microarray and immunohistochemistry was used. Methods: To determine the physiological importance, we used RCC4 stable cell line derived from clear cell renal cell carcinoma, where apoptosis induction by a mixture of five chemotherapeutics with/without silencing of H2S-producing enzymes was detected. Immunofluorescence was used to determine each enzyme in the cells. Results: In clear cell renal cell carcinomas, expression of H2S-producing enzymes was mostly decreased compared to a part of kidney that was distal from the tumor. To evaluate a potential role of H2S-producing enzymes in the apoptosis induction, we used RCC4 stable cell line. We have found that silencing of cystathionine-beta-synthase and cystathionine.-lyase prevented induction of apoptosis. Immunofluorescence staining clearly showed that these enzymes were upregulated during apoptosis in RCC4 cells. Conclusion: Based on these results we concluded that in clear cell renal cell carcinoma, reduced expression of the H2S-producing enzymes, mainly cystathionine gamma-lyase, might contribute to a resistance to the induction of apoptosis. Increased production of the endogenous H2S, or donation from the external sources might be of a therapeutic importance in these tumors.

Citovat

BREZA, Jan, Andrea SOLTYSOVA, Sona HUDECOVA, Adela PENESOVA, Ivan SZADVÁRI, Petr BABULA, Barbora CHOVANCOVA, Lubomira LENCESOVA, Ondrej POS, Jan BREZA, Karol ONDRIAS and Oľga KRIŽANOVÁ. Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma. BMC Cancer. London: BioMed Central, 2018, vol. 18, No 591, p. 1-8. ISSN 1471-2407. Available from: https://dx.doi.org/10.1186/s12885-018-4508-1.

@article{1453617,
   author = {Breza, Jan and Soltysova, Andrea and Hudecova, Sona and Penesova, Adela and Szadvári, Ivan and Babula, Petr and Chovancova, Barbora and Lencesova, Lubomira and Pos, Ondrej and Breza, Jan and Ondrias, Karol and Križanová, Oľga},
   article_location = {London},
   article_number = {591},
   doi = {http://dx.doi.org/10.1186/s12885-018-4508-1},
   keywords = {Cystathionine-beta-synthase; Cystathionine gamma-lyase; 3-Mercaptopyruvate sulfurtransferase; Apoptosis; Clear cell renal cell carcinoma},
   language = {eng},
   issn = {1471-2407},
   journal = {BMC Cancer},
   title = {Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma},
   url = {http://dx.doi.org/10.1186/s12885-018-4508-1},
   volume = {18},
   year = {2018}
}

TY  - JOUR
ID  - 1453617
AU  - Breza, Jan - Soltysova, Andrea - Hudecova, Sona - Penesova, Adela - Szadvári, Ivan - Babula, Petr - Chovancova, Barbora - Lencesova, Lubomira - Pos, Ondrej - Breza, Jan - Ondrias, Karol - Križanová, Oľga
PY  - 2018
TI  - Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma
JF  - BMC Cancer
VL  - 18
IS  - 591
SP  - 1-8
EP  - 1-8
PB  - BioMed Central
SN  - 14712407
KW  - Cystathionine-beta-synthase
KW  - Cystathionine gamma-lyase
KW  - 3-Mercaptopyruvate sulfurtransferase
KW  - Apoptosis
KW  - Clear cell renal cell carcinoma
UR  - http://dx.doi.org/10.1186/s12885-018-4508-1
N2  - Background: Knowledge about the expression and thus a role of enzymes that produce endogenous H2S-cystathionine-beta-synthase, cystathionine gamma-lyase and mercaptopyruvate sulfurtransferase - in renal tumors is still controversial. In this study we aimed to determine the expression of these enzymes relatively to the expression in unaffected part of kidney from the same patient and to found relation of these changes to apoptosis. To evaluate patient's samples, microarray and immunohistochemistry was used. Methods: To determine the physiological importance, we used RCC4 stable cell line derived from clear cell renal cell carcinoma, where apoptosis induction by a mixture of five chemotherapeutics with/without silencing of H2S-producing enzymes was detected. Immunofluorescence was used to determine each enzyme in the cells. Results: In clear cell renal cell carcinomas, expression of H2S-producing enzymes was mostly decreased compared to a part of kidney that was distal from the tumor. To evaluate a potential role of H2S-producing enzymes in the apoptosis induction, we used RCC4 stable cell line. We have found that silencing of cystathionine-beta-synthase and cystathionine.-lyase prevented induction of apoptosis. Immunofluorescence staining clearly showed that these enzymes were upregulated during apoptosis in RCC4 cells. Conclusion: Based on these results we concluded that in clear cell renal cell carcinoma, reduced expression of the H2S-producing enzymes, mainly cystathionine gamma-lyase, might contribute to a resistance to the induction of apoptosis. Increased production of the endogenous H2S, or donation from the external sources might be of a therapeutic importance in these tumors.
ER  -

BREZA, Jan, Andrea SOLTYSOVA, Sona HUDECOVA, Adela PENESOVA, Ivan SZADVÁRI, Petr BABULA, Barbora CHOVANCOVA, Lubomira LENCESOVA, Ondrej POS, Jan BREZA, Karol ONDRIAS and Oľga KRIŽANOVÁ. Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma. \textit{BMC Cancer}. London: BioMed Central, 2018, vol.~18, No~591, p.~1-8. ISSN~1471-2407. Available from: https://dx.doi.org/10.1186/s12885-018-4508-1.

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