Metabolic responses of terrestrial macrolichens to nickel

J 2018

Metabolic responses of terrestrial macrolichens to nickel

KOVÁČIK, Jozef, Sławomir DRESLER and Petr BABULA

Basic information

Original name

Metabolic responses of terrestrial macrolichens to nickel

Authors

KOVÁČIK, Jozef (703 Slovakia, guarantor), Sławomir DRESLER (616 Poland) and Petr BABULA (203 Czech Republic, belonging to the institution)

Edition

Plant Physiology and Biochemistry, Paris, Elsevier, 2018, 0981-9428

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.404

RIV identification code

RIV/00216224:14110/18:00104159

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.plaphy.2018.03.006

UT WoS

000434751400004

Keywords in English

Antioxidants; Heavy metals; Organic acids; Oxidative stress; Reactive oxygen species (ROS); Thiols

Abstract

V originále

Short-term (24 h) responses of Cladonia arbuscula subsp. mitis (formerly known as Cladina and this name is used to distinguish the tested species) and Cladonia furcata to nickel (Ni-2(+)) excess (10 or 100 mu M) were compared. Cladonia accumulated more Ni at higher Ni dose (1.717 mg total Ni/g DW), K amount was unaffected and Ca amount decreased in Cladina only. Fluorescence microscopy detection of total/general ROS and hydrogen peroxide showed Ni-stimulated increase in both species being more pronounced in Cladonia and in mycobiont partner mainly. Nitric oxide visualization (diaminonaphthalene staining) also revealed elevation in response to Ni that could contribute to synthesis of protective metabolites: they may include ascorbic acid or reduced glutathione which increased in Ni-exposed Cladina or Cladonia, respectively. Only low content of phytochelatin 2 was detected in Ni-treated Cladonia and the role in Ni chelation is not apparent. Among aliphatic organic acids, content of citric or succinic acid was not or slightly affected by Ni, production of malic acid dropped by ca. 50% in both species and alpha-ketoglutaric acid showed the opposite behavior in the tested species. Data indicate that even short-term Ni treatments induce metabolic changes and symptoms of oxidative stress in lichens, confirming that nickel is not non-toxic metal as frequently visible from standard biochemical assays of basic physiology. Ascorbic acid and GSH rather than aliphatic organic acids seem to contribute to Ni tolerance.

Citovat

KOVÁČIK, Jozef, Sławomir DRESLER and Petr BABULA. Metabolic responses of terrestrial macrolichens to nickel. Plant Physiology and Biochemistry. Paris: Elsevier, 2018, vol. 127, JUN 2018, p. 32-38. ISSN 0981-9428. Available from: https://dx.doi.org/10.1016/j.plaphy.2018.03.006.

@article{1454296,
   author = {Kováčik, Jozef and Dresler, Sławomir and Babula, Petr},
   article_location = {Paris},
   article_number = {JUN 2018},
   doi = {http://dx.doi.org/10.1016/j.plaphy.2018.03.006},
   keywords = {Antioxidants; Heavy metals; Organic acids; Oxidative stress; Reactive oxygen species (ROS); Thiols},
   language = {eng},
   issn = {0981-9428},
   journal = {Plant Physiology and Biochemistry},
   title = {Metabolic responses of terrestrial macrolichens to nickel},
   volume = {127},
   year = {2018}
}

TY  - JOUR
ID  - 1454296
AU  - Kováčik, Jozef - Dresler, Sławomir - Babula, Petr
PY  - 2018
TI  - Metabolic responses of terrestrial macrolichens to nickel
JF  - Plant Physiology and Biochemistry
VL  - 127
IS  - JUN 2018
SP  - 32-38
EP  - 32-38
PB  - Elsevier
SN  - 09819428
KW  - Antioxidants
KW  - Heavy metals
KW  - Organic acids
KW  - Oxidative stress
KW  - Reactive oxygen species (ROS)
KW  - Thiols
N2  - Short-term (24 h) responses of Cladonia arbuscula subsp. mitis (formerly known as Cladina and this name is used to distinguish the tested species) and Cladonia furcata to nickel (Ni-2(+)) excess (10 or 100 mu M) were compared. Cladonia accumulated more Ni at higher Ni dose (1.717 mg total Ni/g DW), K amount was unaffected and Ca amount decreased in Cladina only. Fluorescence microscopy detection of total/general ROS and hydrogen peroxide showed Ni-stimulated increase in both species being more pronounced in Cladonia and in mycobiont partner mainly. Nitric oxide visualization (diaminonaphthalene staining) also revealed elevation in response to Ni that could contribute to synthesis of protective metabolites: they may include ascorbic acid or reduced glutathione which increased in Ni-exposed Cladina or Cladonia, respectively. Only low content of phytochelatin 2 was detected in Ni-treated Cladonia and the role in Ni chelation is not apparent. Among aliphatic organic acids, content of citric or succinic acid was not or slightly affected by Ni, production of malic acid dropped by ca. 50% in both species and alpha-ketoglutaric acid showed the opposite behavior in the tested species. Data indicate that even short-term Ni treatments induce metabolic changes and symptoms of oxidative stress in lichens, confirming that nickel is not non-toxic metal as frequently visible from standard biochemical assays of basic physiology. Ascorbic acid and GSH rather than aliphatic organic acids seem to contribute to Ni tolerance.
ER  -

KOVÁČIK, Jozef, Sławomir DRESLER and Petr BABULA. Metabolic responses of terrestrial macrolichens to nickel. \textit{Plant Physiology and Biochemistry}. Paris: Elsevier, 2018, vol.~127, JUN 2018, p.~32-38. ISSN~0981-9428. Available from: https://dx.doi.org/10.1016/j.plaphy.2018.03.006.

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