KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ and Petr BABULA. Metal-induced oxidative stress in terrestrial macrolichens. Chemosphere. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, vol. 203, JUL 2018, p. 402-409. ISSN 0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2018.03.112.
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Basic information
Original name Metal-induced oxidative stress in terrestrial macrolichens
Authors KOVÁČIK, Jozef (703 Slovakia, guarantor), Sławomir DRESLER (616 Poland), Viera PETERKOVÁ (703 Slovakia) and Petr BABULA (203 Czech Republic, belonging to the institution).
Edition Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2018, 0045-6535.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30105 Physiology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 5.108
RIV identification code RIV/00216224:14110/18:00104164
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1016/j.chemosphere.2018.03.112
UT WoS 000432235000046
Keywords in English Antioxidants; Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress; Reactive oxygen species (ROS)
Tags 14110515, rivok
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 10/2/2019 16:00.
Abstract
Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcate to copper (CuII) or chromium (CrIII) excess (10 or 100 mu M) were compared. C arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens. (C) 2018 Elsevier Ltd. All rights reserved.
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