Metal-induced oxidative stress in terrestrial macrolichens

J 2018

Metal-induced oxidative stress in terrestrial macrolichens

KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ and Petr BABULA

Basic information

Original name

Metal-induced oxidative stress in terrestrial macrolichens

Authors

KOVÁČIK, Jozef (703 Slovakia, guarantor), Sławomir DRESLER (616 Poland), Viera PETERKOVÁ (703 Slovakia) and Petr BABULA (203 Czech Republic, belonging to the institution)

Edition

Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2018, 0045-6535

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.108

RIV identification code

RIV/00216224:14110/18:00104164

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.chemosphere.2018.03.112

UT WoS

000432235000046

Keywords in English

Antioxidants; Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress; Reactive oxygen species (ROS)

Abstract

V originále

Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcate to copper (CuII) or chromium (CrIII) excess (10 or 100 mu M) were compared. C arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens. (C) 2018 Elsevier Ltd. All rights reserved.

Citovat

KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ and Petr BABULA. Metal-induced oxidative stress in terrestrial macrolichens. Chemosphere. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, vol. 203, JUL 2018, p. 402-409. ISSN 0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2018.03.112.

@article{1454396,
   author = {Kováčik, Jozef and Dresler, Sławomir and Peterková, Viera and Babula, Petr},
   article_location = {OXFORD},
   article_number = {JUL 2018},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2018.03.112},
   keywords = {Antioxidants; Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress; Reactive oxygen species (ROS)},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Metal-induced oxidative stress in terrestrial macrolichens},
   volume = {203},
   year = {2018}
}

TY  - JOUR
ID  - 1454396
AU  - Kováčik, Jozef - Dresler, Sławomir - Peterková, Viera - Babula, Petr
PY  - 2018
TI  - Metal-induced oxidative stress in terrestrial macrolichens
JF  - Chemosphere
VL  - 203
IS  - JUL 2018
SP  - 402-409
EP  - 402-409
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00456535
KW  - Antioxidants
KW  - Bioaccumulation
KW  - Fluorescence microscopy
KW  - Heavy metals
KW  - Oxidative stress
KW  - Reactive oxygen species (ROS)
N2  - Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcate to copper (CuII) or chromium (CrIII) excess (10 or 100 mu M) were compared. C arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens. (C) 2018 Elsevier Ltd. All rights reserved.
ER  -

KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ and Petr BABULA. Metal-induced oxidative stress in terrestrial macrolichens. \textit{Chemosphere}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, vol.~203, JUL 2018, p.~402-409. ISSN~0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2018.03.112.

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