J 2018

Bilateral activation of STAT3 by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and its nuclear translocation in primary sensory neurons following unilateral sciatic nerve injury

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Viktória KOKOŠOVÁ, Václav BRÁZDA et. al.

Basic information

Original name

Bilateral activation of STAT3 by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and its nuclear translocation in primary sensory neurons following unilateral sciatic nerve injury

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor, belonging to the institution), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution), Viktória KOKOŠOVÁ (703 Slovakia, belonging to the institution), Václav BRÁZDA (203 Czech Republic, belonging to the institution) and Marek JOUKAL (203 Czech Republic, belonging to the institution)

Edition

Histochemistry and Cell Biology, Heidelberg, Springer, 2018, 0948-6143

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30106 Anatomy and morphology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.640

RIV identification code

RIV/00216224:14110/18:00101282

Organization unit

Faculty of Medicine

UT WoS

000436854000004

Keywords in English

Dorsal root ganglia; Neuroinflammation; Systemic reaction; Peripheral nerve injury

Tags

Tags

International impact, Reviewed
Změněno: 9/2/2019 21:06, Soňa Böhmová

Abstract

V originále

Unilateral sciatic nerve compression (SNC) or complete sciatic nerve transection (CSNT), both varying degrees of nerve injury, induced activation of STAT3 bilaterally in the dorsal root ganglia (DRG) neurons of lumbar (L4-L5) as well as cervical (C6-C8) spinal cord segments. STAT3 activation was by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and was followed by their nuclear translocation. This is the first evidence of STAT3(S727) activation together with the well-known activation of STAT3(Y705) in primary sensory neurons upon peripheral nerve injury. Bilateral activation of STAT3 in DRG neurons of spinal segments anatomically both associated as well as non-associated with the injured nerve indicates diffusion of STAT3 activation inducers along the spinal cord. Increased levels of IL-6 protein in the CSF following nerve injury as well as activation and nuclear translocation of STAT3 in DRG after intrathecal injection of IL-6 shows that this cytokine, released into the subarachnoid space can penetrate the DRG to activate STAT3. Previous results on increased bilateral IL-6 synthesis and the present manifestation of STAT3 activation in remote DRG following unilateral sciatic nerve injury may reflect a systemic reaction of the DRG neurons to nerve injury.

Links

GA16-08508S, research and development project
Name: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem (Acronym: ERP)
Investor: Czech Science Foundation