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@article{1454698,
author = {Pospíšilová, Petra and Grange, Philippe Alain and Grillová, Linda and Paštěková, Lenka and Martinet, Pervenche and Janier, Michel and Vermersch, Annie and Benhaddou, Nadjet and Del Giudice, Pascal and Alcaraz, Isabelle and Truchetet, Francois and Dupin, Nicolas and Šmajs, David},
article_location = {San Francisco},
article_number = {7},
doi = {http://dx.doi.org/10.1371/journal.pone.0201068},
keywords = {Treponema pallidum},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Multi-locus sequence typing of Treponema pallidum subsp pallidum present in clinical samples from France: Infecting treponemes are genetically diverse and belong to 18 allelic profiles},
volume = {13},
year = {2018}
}
TY - JOUR
ID - 1454698
AU - Pospíšilová, Petra - Grange, Philippe Alain - Grillová, Linda - Paštěková, Lenka - Martinet, Pervenche - Janier, Michel - Vermersch, Annie - Benhaddou, Nadjet - Del Giudice, Pascal - Alcaraz, Isabelle - Truchetet, Francois - Dupin, Nicolas - Šmajs, David
PY - 2018
TI - Multi-locus sequence typing of Treponema pallidum subsp pallidum present in clinical samples from France: Infecting treponemes are genetically diverse and belong to 18 allelic profiles
JF - Plos one
VL - 13
IS - 7
SP - 1-17
EP - 1-17
PB - Public Library of Science
SN - 19326203
KW - Treponema pallidum
N2 - Treponema pallidum subsp. pallidum, the causative agent of sexually transmitted syphilis, detected in clinical samples from France, was subjected to molecular typing using the recently developed Multilocus Sequence Typing system. The samples (n = 133) used in this study were collected from 2010-2016 from patients with diagnosed primary or secondary syphilis attending outpatient centers or hospitals in several locations in France. Altogether, 18 different allelic profiles were found among the fully typed samples (n = 112). There were five allelic variants identified for TP0136, 12 for TP0548, and eight for TP0705. Out of the identified alleles, one, seven, and three novel alleles were identified in TP0136, TP0548, and TP0705, respectively. Partial allelic profiles were obtained from 6 samples. The majority of samples (n = 110) belonged to the SS14-like cluster of TPA isolates while 7 clustered with Nichols-like isolates. Patients infected with Nichols-like samples were more often older (p = 0.041) and more often diagnosed with secondary syphilis (p = 0.033) compared to patients infected with SS14-like samples. In addition, macrolide resistance caused by the A2058G mutation was found to be associated with allelic profile 1.3.1 or with strains belonging to the 1.3.1 lineage (p<0.001). The genetic diversity among TPA strains infecting the European population was surprisingly high, which suggests that additional studies are needed to reveal the full genetic diversity of TPA pathogens infecting humans.
ER -
POSPÍŠILOVÁ, Petra, Philippe Alain GRANGE, Linda GRILLOVÁ, Lenka PAŠTĚKOVÁ, Pervenche MARTINET, Michel JANIER, Annie VERMERSCH, Nadjet BENHADDOU, Pascal DEL GIUDICE, Isabelle ALCARAZ, Francois TRUCHETET, Nicolas DUPIN and David ŠMAJS. Multi-locus sequence typing of Treponema pallidum subsp pallidum present in clinical samples from France: Infecting treponemes are genetically diverse and belong to 18 allelic profiles. \textit{Plos one}. San Francisco: Public Library of Science, 2018, vol.~13, No~7, p.~1-17. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0201068.
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